PMID- 24134932
OWN - NLM
STAT- MEDLINE
DCOM- 20140124
LR  - 20210208
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 44
IP  - 12
DP  - 2013 Dec
TI  - 9p21 gene locus in Spitz nevi of older individuals: absence of cytogenetic and 
      immunohistochemical findings associated with malignancy.
PG  - 2822-8
LID - S0046-8177(13)00323-7 [pii]
LID - 10.1016/j.humpath.2013.07.034 [doi]
AB  - The diagnosis of Spitz nevus in an elderly individual is often met with 
      skepticism because the lesion can be difficult to distinguish from melanoma and 
      because the probability of a malignant melanoma is higher in older patients. 
      Recently, increased sensitivity for detection of malignant spitzoid neoplasms 
      using 9p21 fluorescence in situ hybridization (FISH) has been described. In this 
      study, we address the question of whether histopathologically typical Spitz nevi 
      occurring in patients 50 years and older show any abnormalities regarding the 
      9p21 CDKN2A tumor suppressor gene locus. p16 immunohistochemistry (IHC), as well 
      as dual-color FISH for assessment of diploid or hypodiploid status at 9p21, was 
      performed in 25 classic Spitz nevi from patients 50 years and older and was 
      compared with findings in a younger control population. All cases of typical 
      Spitz nevi occurring in older patients retained p16 expression by 
      immunohistochemistry and showed normal, diploid 9p21 FISH signals. Heterozygous 
      loss of 9p21 by FISH was noted in a control case of a 9-year-old girl and is of 
      unknown significance. These findings indicate that p16 expression by 
      immunohistochemistry in classic Spitz nevi correlates well with absence of 
      malignancy-associated cytogenetic abnormalities at 9p21 by FISH independent of 
      the patient's age. Assessment of p16 expression by standard immunohistochemistry 
      may therefore be reassuring in routine clinical practice when the patient is of 
      advanced age, and can be helpful as a screening tool to select IHC-negative cases 
      for extended FISH analysis targeting the 9p21 gene locus.
CI  - (c) 2013.
FAU - Horst, Basil A
AU  - Horst BA
AD  - Department of Dermatology, Columbia University Medical Center, New York City, NY 
      10032, USA; Department of Pathology & Cell Biology, Columbia University Medical 
      Center, New York City, NY 10032, USA. Electronic address: bh2179@columbia.edu.
FAU - Terrano, David
AU  - Terrano D
FAU - Fang, Yuqiang
AU  - Fang Y
FAU - Silvers, David N
AU  - Silvers DN
FAU - Busam, Klaus J
AU  - Busam KJ
LA  - eng
PT  - Journal Article
DEP - 20131014
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (CDKN2A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Chromosome Aberrations
MH  - Cyclin-Dependent Kinase Inhibitor p16
MH  - Diagnosis, Differential
MH  - Female
MH  - Genes, p16
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Melanoma/*diagnosis/genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Proteins/metabolism
MH  - Nevus, Epithelioid and Spindle Cell/*diagnosis/genetics/metabolism
MH  - Skin Neoplasms/*diagnosis/genetics/metabolism
OTO - NOTNLM
OT  - 9p21 FISH
OT  - CDKN2A
OT  - Spitz nevus
OT  - Spitzoid melanoma
OT  - p16 IHC
EDAT- 2013/10/19 06:00
MHDA- 2014/01/25 06:00
CRDT- 2013/10/19 06:00
PHST- 2013/06/12 00:00 [received]
PHST- 2013/07/25 00:00 [revised]
PHST- 2013/07/26 00:00 [accepted]
PHST- 2013/10/19 06:00 [entrez]
PHST- 2013/10/19 06:00 [pubmed]
PHST- 2014/01/25 06:00 [medline]
AID - S0046-8177(13)00323-7 [pii]
AID - 10.1016/j.humpath.2013.07.034 [doi]
PST - ppublish
SO  - Hum Pathol. 2013 Dec;44(12):2822-8. doi: 10.1016/j.humpath.2013.07.034. Epub 2013 
      Oct 14.