PMID- 24134933
OWN - NLM
STAT- MEDLINE
DCOM- 20140124
LR  - 20201209
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 44
IP  - 12
DP  - 2013 Dec
TI  - Analysis of MAML2 rearrangement in mucoepidermoid carcinoma of the thymus.
PG  - 2799-805
LID - S0046-8177(13)00320-1 [pii]
LID - 10.1016/j.humpath.2013.07.031 [doi]
AB  - Primary thymic mucoepidermoid carcinoma (TMEC) is rare. High-grade TMEC can be 
      difficult to distinguish from poorly differentiated squamous cell carcinoma and 
      adenosquamous carcinoma. A strong association between mucoepidermoid carcinoma 
      (MEC) and t(11;19)(q21;p13) has been observed in other anatomical sites. Although 
      this translocation is largely considered a disease-defining event for MEC, its 
      incidence in TMEC has not been explored. In this study, we evaluate the value of 
      identifying MAML2 rearrangement by fluorescence in situ hybridization (FISH) to 
      distinguish TMEC from poorly differentiated squamous cell carcinoma and 
      adenosquamous carcinoma. Cases of TMEC, moderate to poorly differentiated 
      squamous cell carcinoma, and adenosquamous carcinoma were re-reviewed by 3 
      surgical pathologists and classified according to the current World Health 
      Organization classification of thymic tumors (2004). Cases of TMEC were 
      histologically graded using the Brandwein system. FISH was used to detect MAML2 
      rearrangements using a break-apart probe. FISH for MAML2 rearrangement was 
      performed on cases of TMEC (n = 2), thymic squamous cell carcinoma (n = 5), and 
      thymic adenosquamous carcinoma (n = 3). The 2 cases of TMEC showed MAML2 
      rearrangement. All other tested cases did not show rearrangement of MAML2. In 
      conclusion, using FISH to identify MAML2 rearrangement is a valuable diagnostic 
      tool in the evaluation of thymic malignancies, specifically, distinguishing TMEC 
      from squamous cell carcinoma and adenosquamous carcinoma. These findings also 
      suggest that TMEC has both histomorphologic and cytogenetic similarities to cases 
      of MEC arising from other anatomical sites.
CI  - (c) 2013.
FAU - Roden, Anja C
AU  - Roden AC
AD  - Department of Laboratory Medicine & Pathology, Mayo Clinic Rochester, Rochester 
      55905, MN, USA. Electronic address: Roden.anja@mayo.edu.
FAU - Erickson-Johnson, Michele R
AU  - Erickson-Johnson MR
FAU - Yi, Eunhee S
AU  - Yi ES
FAU - Garcia, Joaquin J
AU  - Garcia JJ
LA  - eng
PT  - Journal Article
DEP - 20131014
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MAML2 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Adenosquamous/*diagnosis/genetics/pathology
MH  - Carcinoma, Mucoepidermoid/*diagnosis/genetics/pathology
MH  - Carcinoma, Squamous Cell/*diagnosis/genetics/pathology
MH  - DNA-Binding Proteins/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nuclear Proteins/*genetics
MH  - Thymus Neoplasms/*diagnosis/genetics/pathology
MH  - Trans-Activators
MH  - Transcription Factors/*genetics
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - MAML2
OT  - Mucoepidermoid carcinoma
OT  - Thymic carcinoma
EDAT- 2013/10/19 06:00
MHDA- 2014/01/25 06:00
CRDT- 2013/10/19 06:00
PHST- 2013/04/17 00:00 [received]
PHST- 2013/07/25 00:00 [revised]
PHST- 2013/07/26 00:00 [accepted]
PHST- 2013/10/19 06:00 [entrez]
PHST- 2013/10/19 06:00 [pubmed]
PHST- 2014/01/25 06:00 [medline]
AID - S0046-8177(13)00320-1 [pii]
AID - 10.1016/j.humpath.2013.07.031 [doi]
PST - ppublish
SO  - Hum Pathol. 2013 Dec;44(12):2799-805. doi: 10.1016/j.humpath.2013.07.031. Epub 
      2013 Oct 14.