PMID- 24136204
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20211021
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 15
IP  - 1
DP  - 2014
TI  - Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses 
      induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 
      DNA vaccine in swine.
PG  - 99-109
AB  - The purpose of this study was to investigate the effects of porcine interleukin 
      (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive 
      and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression 
      plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then 
      expressed in Marc-145 cells. The effects of these genes were detected using an 
      indirect immunofluorescent assay and reverse transcription polymerase chain 
      reaction (RT-PCR). Characteristic fluorescence was observed at different times 
      after pcDNA- ORF5 was expressed in the Marc-145 cells, and PCR products 
      corresponding to ORF5, IL-2, and IL-4 genes were detected at 48 h. Based on these 
      data, healthy piglets were injected intramuscularly with different combinations 
      of the purified plasmids: pcDNA-ORF5 alone, pcDNA-ORF5 + pcDNA-IL-2, pcDNA-ORF5 + 
      pcDNA-IL-4, and pcDNA-ORF5 + pcDNA- IL-4 + pcDNA-IL-2. The ensuing humoral immune 
      responses, percentages of CD4(+) and CD8(+) T lymphocytes, proliferation indices, 
      and interferon-g expression were analyzed. Results revealed that the piglets 
      co-immunized with pcDNA-ORF5 + pcDNA-IL-4 + pcDNA-IL-2 plasmids developed 
      significantly higher antibody titers and neutralizing antibody levels, had 
      significantly increased levels of specific T lymphocyte proliferation, elevated 
      percentages of CD4(+) and CD8(+) T lymphocytes, and significantly higher IFN-gamma 
      production than the other inoculated pigs (p < 0.05).
FAU - Tang, Deyuan
AU  - Tang D
AD  - Department of Animal Science, Guizhou University, Guiyang 550025, 
      China.tdyuan@163.com.
FAU - Liu, Jian
AU  - Liu J
FAU - Li, Chunyan
AU  - Li C
FAU - Zhang, Hua
AU  - Zhang H
FAU - Ma, Ping
AU  - Ma P
FAU - Luo, Xianfeng
AU  - Luo X
FAU - Zeng, Zhiyong
AU  - Zeng Z
FAU - Hong, Nining
AU  - Hong N
FAU - Liu, Xia
AU  - Liu X
FAU - Wang, Bin
AU  - Wang B
FAU - Wang, Feng
AU  - Wang F
FAU - Gan, Zhenlei
AU  - Gan Z
FAU - Hao, Fei
AU  - Hao F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131018
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Interleukin-2)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Escherichia coli/genetics
MH  - Haplorhini
MH  - Immunity, Cellular
MH  - Interleukin-2/genetics/*metabolism
MH  - Interleukin-4/genetics/*metabolism
MH  - Neutralization Tests/veterinary
MH  - Plasmids
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Recombinant Proteins/genetics/metabolism
MH  - Swine
MH  - Vaccines, DNA/immunology
MH  - Viral Envelope Proteins/*genetics/metabolism
MH  - Viral Vaccines/*immunology
PMC - PMC3973771
OTO - NOTNLM
OT  - IL-2
OT  - IL-4
OT  - ORF5 DNA vaccine
OT  - immunogenicity
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2013/10/19 06:00
MHDA- 2014/12/15 06:00
PMCR- 2014/03/01
CRDT- 2013/10/19 06:00
PHST- 2012/11/12 00:00 [received]
PHST- 2013/07/09 00:00 [accepted]
PHST- 2013/10/19 06:00 [entrez]
PHST- 2013/10/19 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - jvs.2013.070 [pii]
AID - 10.4142/jvs.2014.15.1.99 [doi]
PST - ppublish
SO  - J Vet Sci. 2014;15(1):99-109. doi: 10.4142/jvs.2014.15.1.99. Epub 2013 Oct 18.