PMID- 24136217 OWN - NLM STAT- MEDLINE DCOM- 20141209 LR - 20211203 IS - 1976-555X (Electronic) IS - 1229-845X (Print) IS - 1229-845X (Linking) VI - 15 IP - 1 DP - 2014 TI - Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment. PG - 27-33 AB - In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus. FAU - Yoo, Dae Young AU - Yoo DY AD - Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea. FAU - Kim, Woosuk AU - Kim W FAU - Kim, Dae Won AU - Kim DW FAU - Nam, Sung Min AU - Nam SM FAU - Jung, Hyo Young AU - Jung HY FAU - Kim, Jong Whi AU - Kim JW FAU - Lee, Choong Hyun AU - Lee CH FAU - Choi, Jung Hoon AU - Choi JH FAU - Won, Moo-Ho AU - Won MH FAU - Yoon, Yeo Sung AU - Yoon YS FAU - Hwang, In Koo AU - Hwang IK LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131018 PL - Korea (South) TA - J Vet Sci JT - Journal of veterinary science JID - 100964185 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Dcx protein, mouse) RN - 0 (Doublecortin Domain Proteins) RN - 0 (Doublecortin Protein) RN - 0 (Hypoglycemic Agents) RN - 0 (Ki-67 Antigen) RN - 0 (Microtubule-Associated Proteins) RN - 0 (Neuropeptides) RN - 0 (Thiazolidinediones) RN - 05V02F2KDG (Rosiglitazone) SB - IM MH - Animals MH - Blotting, Western MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cell Differentiation/*drug effects MH - Cell Proliferation/drug effects MH - Dentate Gyrus/growth & development/physiology MH - Diet, Fat-Restricted MH - *Diet, High-Fat MH - Doublecortin Domain Proteins MH - Doublecortin Protein MH - Hippocampus/growth & development/physiology MH - Hypoglycemic Agents/*pharmacology MH - Immunohistochemistry MH - Ki-67 Antigen/metabolism MH - Male MH - Mice, Inbred C57BL MH - Microtubule-Associated Proteins/metabolism MH - Neurogenesis/*drug effects MH - Neuropeptides/metabolism MH - Rosiglitazone MH - Thiazolidinediones/*pharmacology PMC - PMC3973763 OTO - NOTNLM OT - brain-derived neurotrophic factor OT - dentate gyrus OT - high-fat diet OT - rosiglitazone EDAT- 2013/10/19 06:00 MHDA- 2014/12/15 06:00 PMCR- 2014/03/01 CRDT- 2013/10/19 06:00 PHST- 2013/08/06 00:00 [received] PHST- 2013/10/06 00:00 [accepted] PHST- 2013/10/19 06:00 [entrez] PHST- 2013/10/19 06:00 [pubmed] PHST- 2014/12/15 06:00 [medline] PHST- 2014/03/01 00:00 [pmc-release] AID - jvs.2013.083 [pii] AID - 10.4142/jvs.2014.15.1.27 [doi] PST - ppublish SO - J Vet Sci. 2014;15(1):27-33. doi: 10.4142/jvs.2014.15.1.27. Epub 2013 Oct 18.