PMID- 24136966
OWN - NLM
STAT- MEDLINE
DCOM- 20131127
LR  - 20240216
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 342
IP  - 6156
DP  - 2013 Oct 18
TI  - Genomically recoded organisms expand biological functions.
PG  - 357-60
LID - 10.1126/science.1241459 [doi]
AB  - We describe the construction and characterization of a genomically recoded 
      organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 
      with synonymous UAA codons, which permitted the deletion of release factor 1 and 
      reassignment of UAG translation function. This GRO exhibited improved properties 
      for incorporation of nonstandard amino acids that expand the chemical diversity 
      of proteins in vivo. The GRO also exhibited increased resistance to T7 
      bacteriophage, demonstrating that new genetic codes could enable increased viral 
      resistance.
FAU - Lajoie, Marc J
AU  - Lajoie MJ
AD  - Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
FAU - Rovner, Alexis J
AU  - Rovner AJ
FAU - Goodman, Daniel B
AU  - Goodman DB
FAU - Aerni, Hans-Rudolf
AU  - Aerni HR
FAU - Haimovich, Adrian D
AU  - Haimovich AD
FAU - Kuznetsov, Gleb
AU  - Kuznetsov G
FAU - Mercer, Jaron A
AU  - Mercer JA
FAU - Wang, Harris H
AU  - Wang HH
FAU - Carr, Peter A
AU  - Carr PA
FAU - Mosberg, Joshua A
AU  - Mosberg JA
FAU - Rohland, Nadin
AU  - Rohland N
FAU - Schultz, Peter G
AU  - Schultz PG
FAU - Jacobson, Joseph M
AU  - Jacobson JM
FAU - Rinehart, Jesse
AU  - Rinehart J
FAU - Church, George M
AU  - Church GM
FAU - Isaacs, Farren J
AU  - Isaacs FJ
LA  - eng
GR  - T32GM07205/GM/NIGMS NIH HHS/United States
GR  - K01DK089006/DK/NIDDK NIH HHS/United States
GR  - DP5 OD009172/OD/NIH HHS/United States
GR  - 27306C0002/ES/NIEHS NIH HHS/United States
GR  - K01 DK089006/DK/NIDDK NIH HHS/United States
GR  - T32 GM007205/GM/NIGMS NIH HHS/United States
GR  - 1DP5OD009172-01/OD/NIH HHS/United States
GR  - 27304C0002/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Amino Acids)
RN  - 0 (Codon, Terminator)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Peptide Termination Factors)
SB  - IM
MH  - Amino Acid Substitution/genetics
MH  - Amino Acids/*genetics
MH  - Bacteriophage T7/*physiology
MH  - Codon, Terminator/*genetics
MH  - Escherichia coli/*genetics/*virology
MH  - Escherichia coli Proteins/genetics
MH  - Genetic Engineering
MH  - Genome, Bacterial
MH  - Organisms, Genetically Modified/*genetics/*virology
MH  - Peptide Chain Termination, Translational/genetics
MH  - Peptide Termination Factors/genetics
PMC - PMC4924538
MID - NIHMS606579
EDAT- 2013/10/19 06:00
MHDA- 2013/12/16 06:00
PMCR- 2016/06/28
CRDT- 2013/10/19 06:00
PHST- 2013/10/19 06:00 [entrez]
PHST- 2013/10/19 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2016/06/28 00:00 [pmc-release]
AID - 342/6156/357 [pii]
AID - 10.1126/science.1241459 [doi]
PST - ppublish
SO  - Science. 2013 Oct 18;342(6156):357-60. doi: 10.1126/science.1241459.