PMID- 24137257
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 6
IP  - 3
DP  - 2013 Sep
TI  - Application of clinical PET imaging to human laryngeal squamous cell carcinoma 
      xenografts.
PG  - 737-742
AB  - Positron emission tomography (PET) imaging with [F-18]-fluoro-2-deoxy-D-glucose 
      ((18)F-FDG) is extensively applied in clinical practice. However, in animal 
      experiments, the application of clinical PET is difficult, due to limitations in 
      sensitivity and spatial resolution. This study aimed to determine the potential 
      of (18)F-FDG PET with regard to the imaging of human laryngeal squamous cell 
      carcinoma (LSCC) xenografts. Twenty-seven LSCC tumor-bearing nude mice were 
      divided randomly into seven groups which were each handled differently; the 
      anesthetization, fasting, warming and the time point at which scanning was 
      initiated were varied. The size of each xenograft was measured prior to 
      conducting the scan. Using the RAMLA 3D image reconstruction method, images were 
      acquired. The region of interest (ROI) technology was adopted to calculate target 
      and non-target (T/N) ratios. The results were subsequently analyzed by 
      semiquantitative analysis. The analysis showed that there was no significant 
      correlation between tumor size and PET image quality (r=0.381, P>0.05); however, 
      the handling conditions of the mice had a greater influence on the tumor image 
      quality. Fasting increased (18)F-FDG uptake (T/N, 1.153+/-0.008) to a certain 
      degree, although the effect was unstable. By contrast, combining warming and 
      fasting increased (18)F-FDG uptake significantly (T/N, 2.0+/-0.29; P<0.05). The 
      acquisition time had no impact on the tumor image quality. The study demonstrated 
      that the application of clinical PET scanning has potential in the study of human 
      LSCC xenografts in nude mice, and that the quality of the image of the tumor is 
      greatly influenced by the handling conditions of the animals.
FAU - Xu, Ou
AU  - Xu O
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China ; Departments of 
      Otorhinolaryngology Head and Neck Surgery, Bethune International Peace Hospital, 
      Shijiazhuang, Hebei 050082, P.R. China.
FAU - Li, Xiaoming
AU  - Li X
FAU - Shan, Chunguang
AU  - Shan C
FAU - Yang, Xing
AU  - Yang X
FAU - Zhang, Lin
AU  - Zhang L
FAU - Wang, Jingmiao
AU  - Wang J
LA  - eng
PT  - Journal Article
DEP - 20130703
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC3786834
OTO - NOTNLM
OT  - animal handling
OT  - laryngeal carcinoma
OT  - nude mice
OT  - positron emission tomography
OT  - xenograft
EDAT- 2013/10/19 06:00
MHDA- 2013/10/19 06:01
PMCR- 2013/07/03
CRDT- 2013/10/19 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/06/10 00:00 [accepted]
PHST- 2013/10/19 06:00 [entrez]
PHST- 2013/10/19 06:00 [pubmed]
PHST- 2013/10/19 06:01 [medline]
PHST- 2013/07/03 00:00 [pmc-release]
AID - etm-06-03-0737 [pii]
AID - 10.3892/etm.2013.1200 [doi]
PST - ppublish
SO  - Exp Ther Med. 2013 Sep;6(3):737-742. doi: 10.3892/etm.2013.1200. Epub 2013 Jul 3.