PMID- 24138587
OWN - NLM
STAT- MEDLINE
DCOM- 20141027
LR  - 20140221
IS  - 1525-6049 (Electronic)
IS  - 0886-022X (Linking)
VI  - 36
IP  - 2
DP  - 2014 Mar
TI  - Effect of mesenteric lymph duct ligation on gene expression profiles of renal 
      tissue in hemorrhagic shock rats with fluid resuscitation.
PG  - 271-7
LID - 10.3109/0886022X.2013.844623 [doi]
AB  - BACKGROUND: Acute kidney injury (AKI), a common pathological process following 
      hemorrhagic shock, can lead to an internal milieu disorder, which is an important 
      factor of multiple organ failure (MOF). It has been shown that the mesenteric 
      lymph return plays a deleterious effect on MOF induced by hemorrhagic shock. In 
      this study, we investigated the effects of mesenteric lymph duct ligation (MLDL) 
      on gene expression profiles of renal tissue following hemorrhagic shock with 
      fluid resuscitation. METHODS: After establishment of hemorrhagic shock model and 
      fluid resuscitation in rats of shock and shock ligation groups, the MLDL was 
      performed in shock ligation group, and only threading under the mesenteric lymph 
      duct in the shock group. Then, the fixed position renal tissue was taken out for 
      homogenate in two groups at 3 h after resuscitation, the total mRNA was 
      extracted, reversely transcribed into cDNAs and marked with Cy3 and Cy5. The 
      cDNAs were subjected for microarray scanning with 12,028 cDNA probes; the 
      differentially expressed genes between two groups were analyzed. RESULTS: In the 
      5812 valid dates of rat genomes transcription, there were 34 known differentially 
      expressed genes between the two groups, of which 11 genes were up-regulated 
      whereas 23 genes were down-regulated by MLDL. These different expressed genes 
      encoding protein function were mainly involved in signal transduction, 
      transcription regulation, metabolism, transport, cell growth, cell cycle, cell 
      adhesion, cell movement, cellular component, and biological process. CONCLUSIONS: 
      The mechanism of MLDL alleviating the AKI aftershock might be associated with up- 
      or down-regulation of the above gene expressions.
FAU - Zhao, Zi-Gang
AU  - Zhao ZG
AD  - Institute of Cell Biology, Zhejiang University , Hangzhou, Zhejiang , PR China .
FAU - Niu, Chun-Yu
AU  - Niu CY
FAU - Qiu, Jing-Fu
AU  - Qiu JF
FAU - Chen, Xiao-Dong
AU  - Chen XD
FAU - Li, Ji-Cheng
AU  - Li JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131021
PL  - England
TA  - Ren Fail
JT  - Renal failure
JID - 8701128
RN  - 0 (DNA, Complementary)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Acute Kidney Injury/*genetics/*metabolism
MH  - Animals
MH  - DNA, Complementary/analysis
MH  - Fluid Therapy
MH  - *Gene Expression
MH  - Kidney/*metabolism
MH  - Ligation
MH  - Lymphatic Vessels/*metabolism
MH  - Mesentery
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA/analysis
MH  - Rats
MH  - Shock, Hemorrhagic/*metabolism/therapy
EDAT- 2013/10/22 06:00
MHDA- 2014/10/28 06:00
CRDT- 2013/10/22 06:00
PHST- 2013/10/22 06:00 [entrez]
PHST- 2013/10/22 06:00 [pubmed]
PHST- 2014/10/28 06:00 [medline]
AID - 10.3109/0886022X.2013.844623 [doi]
PST - ppublish
SO  - Ren Fail. 2014 Mar;36(2):271-7. doi: 10.3109/0886022X.2013.844623. Epub 2013 Oct 
      21.