PMID- 24139888
OWN - NLM
STAT- MEDLINE
DCOM- 20140815
LR  - 20131209
IS  - 1872-7654 (Electronic)
IS  - 0301-2115 (Linking)
VI  - 171
IP  - 2
DP  - 2013 Dec
TI  - Placental leptin in HIV-associated preeclampsia.
PG  - 271-6
LID - S0301-2115(13)00480-6 [pii]
LID - 10.1016/j.ejogrb.2013.09.027 [doi]
AB  - OBJECTIVE: HIV-associated preeclampsia reflects a combination of opposing 
      influences on the immune status. The adipocyte hormone leptin has been implicated 
      in the pathophysiology of preeclampsia and in enhancing immunity. This study is 
      the first, to our knowledge, to determine whether leptin levels in the placenta 
      differ between HIV-associated normotensive and preeclamptic pregnancies. The 
      study also compares leptin levels between the exchange and conducting areas of 
      the placenta. STUDY DESIGN: Pregnant women were recruited antenatally and grouped 
      as follows: normotensive HIV uninfected (n=30), normotensive HIV infected (n=60), 
      preeclamptic HIV uninfected (n=30) and preeclamptic HIV infected (n=60). 
      Anthropometric data were collected and placental leptin was analysed by 
      immunohistochemistry and ELISA. RESULTS: Leptin levels were similar in the 
      central and peripheral regions of the placenta. Leptin immunoreactivity was 
      observed amongst the different trophoblast cell populations. Both ELISA and 
      immunohistochemistry of the placental exchange villi indicated that leptin levels 
      were higher in preeclampsia compared to normotensive pregnancies (p<0.001). HIV 
      status had no effect on leptin levels but levels were higher in participants on 
      highly active antiretroviral treatment (HAART) compared to those on prophylaxis 
      for prevention of mother to child transmission (PMTCT) with normotensive 
      (p=0.006) and preeclamptic (p=0.002) pregnancies. The area of immunostaining was 
      greater in the exchange compared to the conducting villi in HIV infected and 
      uninfected preeclampsia. CONCLUSIONS: This novel study establishes an elevation 
      of leptin in preeclamptic placentae, irrespective of HIV status. Leptin elevation 
      was not focal in that it occurred in both central and peripheral regions of the 
      preeclamptic placenta. This suggests a role of leptin in the pathophysiology of 
      preeclampsia.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Haffejee, Firoza
AU  - Haffejee F
AD  - Optics & Imaging Centre, University of KwaZulu-Natal, Durban, South Africa.
FAU - Naicker, Thajasvarie
AU  - Naicker T
FAU - Singh, Moganavelli
AU  - Singh M
FAU - Moodley, Jagidesa
AU  - Moodley J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131001
PL  - Ireland
TA  - Eur J Obstet Gynecol Reprod Biol
JT  - European journal of obstetrics, gynecology, and reproductive biology
JID - 0375672
RN  - 0 (Leptin)
SB  - IM
MH  - Adult
MH  - Antiretroviral Therapy, Highly Active
MH  - Chorionic Villi/physiopathology
MH  - Female
MH  - HIV Infections/*complications/drug therapy
MH  - Humans
MH  - Leptin/*metabolism
MH  - Placenta/*physiopathology
MH  - Pre-Eclampsia/*physiopathology
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/*physiopathology
OTO - NOTNLM
OT  - HIV infection
OT  - Leptin
OT  - Preeclampsia
EDAT- 2013/10/22 06:00
MHDA- 2014/08/16 06:00
CRDT- 2013/10/22 06:00
PHST- 2013/05/10 00:00 [received]
PHST- 2013/08/27 00:00 [revised]
PHST- 2013/09/22 00:00 [accepted]
PHST- 2013/10/22 06:00 [entrez]
PHST- 2013/10/22 06:00 [pubmed]
PHST- 2014/08/16 06:00 [medline]
AID - S0301-2115(13)00480-6 [pii]
AID - 10.1016/j.ejogrb.2013.09.027 [doi]
PST - ppublish
SO  - Eur J Obstet Gynecol Reprod Biol. 2013 Dec;171(2):271-6. doi: 
      10.1016/j.ejogrb.2013.09.027. Epub 2013 Oct 1.