PMID- 24144749
OWN - NLM
STAT- MEDLINE
DCOM- 20140515
LR  - 20161125
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 33
IP  - 10
DP  - 2013 Oct
TI  - [Effects of parthenolide on high glucose-induced cell proliferation, NF-kappaB 
      activation and MCP-1 expression in rat mesangial cells].
PG  - 1471-3
AB  - OBJECTIVE: To explore the effects of parthenolide (PTL) on high 
      glucose-stimulated cell proliferation, NF-kappaB activation and monocyte 
      chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs). 
      METHODS: MCs were cultured in normal glucose (5.6 mmol/L), high glucose (30 
      mmol/L), or high glucose with PTL. MTT assay was used to detect the cell 
      proliferation. MCP-1 content in the supernatant was measured by ELISA, and the 
      level of IkappaBalpha was detected by Western blotting to reflect NF-kappaB activity. EMSA 
      method was used to measure the activation of NF-kappaB. RESULTS: MC proliferation, 
      MCP-1 expression and NF-kappaB activation were significantly enhanced and the 
      expression of NF-kappaB-binding protein IkappaBalpha was obviously reduced in cells cultured 
      in high glucose. Application of PTL obviously abolished the effects of high 
      glucose. CONCLUSION: PTL can suppress high glucose-stimulated NF-kappaB activation 
      and MCP-1 expression in rat MC. These data provide a theoretical basis for the 
      clinical application of PTL in prevention and control of diabetic nephropathy.
FAU - Xu, Zhaozhong
AU  - Xu Z
AD  - Department of Emergency, Zhujiang Hospital, Southern Medical University, 
      Guangzhou 510282, China. E-mail: xzz1008@163.com.
FAU - Jia, Qianqian
AU  - Jia Q
FAU - Li, Hongyu
AU  - Li H
FAU - Wang, Jiancheng
AU  - Wang J
FAU - Zhao, Yan
AU  - Zhao Y
FAU - Chen, Sijia
AU  - Chen S
FAU - Long, Haibo
AU  - Long H
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Nfkbia protein, rat)
RN  - 0 (Sesquiterpenes)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 2RDB26I5ZB (parthenolide)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/isolation & purification/pharmacology
MH  - Asteraceae/chemistry
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Glucose/administration & dosage/pharmacology
MH  - I-kappa B Proteins/metabolism
MH  - Mesangial Cells/*cytology/metabolism
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/*antagonists & inhibitors/metabolism
MH  - Plants, Medicinal/chemistry
MH  - Rats
MH  - Sesquiterpenes/isolation & purification/*pharmacology
EDAT- 2013/10/23 06:00
MHDA- 2014/05/16 06:00
CRDT- 2013/10/23 06:00
PHST- 2013/10/23 06:00 [entrez]
PHST- 2013/10/23 06:00 [pubmed]
PHST- 2014/05/16 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2013 Oct;33(10):1471-3.