PMID- 24147450
OWN - NLM
STAT- MEDLINE
DCOM- 20140725
LR  - 20131126
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Linking)
VI  - 7
IP  - 11
DP  - 2013 Nov 26
TI  - A paradigm change: efficient transfection of human leukemia cells by 
      stimuli-responsive multicompartment micelles.
PG  - 9621-31
LID - 10.1021/nn402072d [doi]
AB  - The controlled nonviral delivery of genetic material using cationic polymers into 
      cells has been of interest during the past three decades, yet the ideal delivery 
      agent featuring utmost transfection efficiency and low cytotoxicity still has to 
      be developed. Here, we demonstrate that multicompartment micelles from 
      stimuli-responsive triblock terpolymers, polybutadiene-block-poly(methacrylic 
      acid)-block-poly(2-(dimethylamino)ethyl methacrylate) (BMAAD), are promising 
      candidates. The structures exhibit a patchy shell, consisting of amphiphilic 
      (interpolyelectrolyte complexes, MAA and D) and cationic patches (excess D), 
      generating a surface reminiscent to those of certain viruses and capable of 
      undergoing pH-dependent changes in charge stoichiometry. After polyplex formation 
      with plasmid DNA, superior transfection efficiencies can be reached for both 
      adherent cells and human leukemia cells. Compared to the gold standard PEI, 
      remarkable improvements and a number of advantages were identified for this 
      system, including increased cellular uptake and an improved release of the 
      genetic material, accompanied by fast and efficient endosomal escape. 
      Furthermore, high sedimentation rates might be beneficial regarding in vitro 
      applications.
FAU - Rinkenauer, Alexandra C
AU  - Rinkenauer AC
AD  - Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller 
      University Jena , Humboldtstrasse 10, 07743, Jena, Germany.
FAU - Schallon, Anja
AU  - Schallon A
FAU - Gunther, Ulrike
AU  - Gunther U
FAU - Wagner, Michael
AU  - Wagner M
FAU - Betthausen, Eva
AU  - Betthausen E
FAU - Schubert, Ulrich S
AU  - Schubert US
FAU - Schacher, Felix H
AU  - Schacher FH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131028
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Biocompatible Materials)
RN  - 0 (Butadienes)
RN  - 0 (Cations)
RN  - 0 (Methacrylates)
RN  - 0 (Micelles)
RN  - 0 (Polymers)
RN  - 0 (Polymethacrylic Acids)
RN  - 0 (polybutadiene-block-poly(methacrylic acid)-block-poly(2-(dimethylamino)ethyl 
      methacrylate))
SB  - IM
MH  - Biocompatible Materials/chemistry
MH  - Biotechnology/methods
MH  - Butadienes/*chemistry
MH  - Cations
MH  - Cell Line, Tumor
MH  - Cryoelectron Microscopy
MH  - Genetic Vectors
MH  - HEK293 Cells
MH  - Hemolysis
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Jurkat Cells
MH  - Leukemia/*pathology
MH  - Methacrylates/chemistry
MH  - *Micelles
MH  - Microscopy, Electron, Transmission
MH  - Nanotechnology/methods
MH  - Plasmids/metabolism
MH  - Polymers/chemistry
MH  - Polymethacrylic Acids/*chemistry
MH  - Transfection
EDAT- 2013/10/24 06:00
MHDA- 2014/07/26 06:00
CRDT- 2013/10/24 06:00
PHST- 2013/10/24 06:00 [entrez]
PHST- 2013/10/24 06:00 [pubmed]
PHST- 2014/07/26 06:00 [medline]
AID - 10.1021/nn402072d [doi]
PST - ppublish
SO  - ACS Nano. 2013 Nov 26;7(11):9621-31. doi: 10.1021/nn402072d. Epub 2013 Oct 28.