PMID- 24147828
OWN - NLM
STAT- MEDLINE
DCOM- 20141003
LR  - 20140109
IS  - 1556-4029 (Electronic)
IS  - 0022-1198 (Linking)
VI  - 59
IP  - 1
DP  - 2014 Jan
TI  - Preliminary study on diffuse axonal injury by Fourier transform infrared 
      spectroscopy histopathology imaging.
PG  - 231-5
LID - 10.1111/1556-4029.12290 [doi]
AB  - The objective of this study was to evaluate the application of Fourier transform 
      infrared (FTIR) spectroscopy for detecting diffuse axonal injury (DAI) in a mouse 
      model. Brain tissues from DAI mouse model were prepared with H&E, silver, and 
      beta-amyloid precursor protein (beta-APP) immunohistochemistry stains and were also 
      studied with FTIR. The infrared spectrum images showed high absorption of amide 
      II in the subcortical white matter of the experimental mouse brain, while there 
      was no obvious expression of amide II in the control mouse brain. The areas with 
      high absorption of amide II were in the same distribution as the DAI region 
      confirmed by the silver and beta-APP studies. The result suggests that high 
      absorption of amide II correlates with axonal injury. The use of FTIR imaging 
      allows the biochemical changes associated with DAI pathologies to be detected in 
      the tissues, thus providing an important adjunct method to the current 
      conventional pathological diagnostic techniques.
CI  - (c) 2013 American Academy of Forensic Sciences.
FAU - Yang, Tiantong
AU  - Yang T
AD  - Key Laboratory of Evidence Science, China University of Political Science and 
      Law, Ministry of Education, 25 West Tucheng Road, Haidian District, Beijing, 
      100088, China.
FAU - He, Guanglong
AU  - He G
FAU - Zhang, Xiang
AU  - Zhang X
FAU - Chang, Lin
AU  - Chang L
FAU - Zhang, Haidong
AU  - Zhang H
FAU - Ripple, Mary G
AU  - Ripple MG
FAU - Fowler, David R
AU  - Fowler DR
FAU - Li, Ling
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131022
PL  - United States
TA  - J Forensic Sci
JT  - Journal of forensic sciences
JID - 0375370
RN  - 0 (Amides)
RN  - 0 (Amyloid beta-Protein Precursor)
SB  - IM
MH  - Amides/chemistry
MH  - Amyloid beta-Protein Precursor/metabolism
MH  - Animals
MH  - Brain/metabolism/pathology
MH  - Diffuse Axonal Injury/*pathology
MH  - Immunohistochemistry
MH  - Mice
MH  - Models, Animal
MH  - Nerve Degeneration/pathology
MH  - Neurons/pathology
MH  - *Spectroscopy, Fourier Transform Infrared
MH  - Staining and Labeling
OTO - NOTNLM
OT  - Fourier transform infrared spectroscopy
OT  - animal study
OT  - diffuse axonal injury
OT  - forensic pathology
OT  - forensic science
OT  - traumatic brain injury
EDAT- 2013/10/24 06:00
MHDA- 2014/10/04 06:00
CRDT- 2013/10/24 06:00
PHST- 2012/03/27 00:00 [received]
PHST- 2012/11/27 00:00 [revised]
PHST- 2012/12/02 00:00 [accepted]
PHST- 2013/10/24 06:00 [entrez]
PHST- 2013/10/24 06:00 [pubmed]
PHST- 2014/10/04 06:00 [medline]
AID - 10.1111/1556-4029.12290 [doi]
PST - ppublish
SO  - J Forensic Sci. 2014 Jan;59(1):231-5. doi: 10.1111/1556-4029.12290. Epub 2013 Oct 
      22.