PMID- 24150755
OWN - NLM
STAT- MEDLINE
DCOM- 20140124
LR  - 20220311
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 54
IP  - 12
DP  - 2013 Nov 15
TI  - Intravitreally transplanted dental pulp stem cells promote neuroprotection and 
      axon regeneration of retinal ganglion cells after optic nerve injury.
PG  - 7544-56
LID - 10.1167/iovs.13-13045 [doi]
AB  - PURPOSE: To investigate the potential therapeutic benefit of intravitreally 
      implanted dental pulp stem cells (DPSCs) on axotomized adult rat retinal ganglion 
      cells (RGCs) using in vitro and in vivo neural injury models. METHODS: 
      Conditioned media collected from cultured rat DPSCs and bone marrow-derived 
      mesenchymal stem cells (BMSCs) were assayed for nerve growth factor (NGF), 
      brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) secretion 
      using ELISA. DPSCs or BMSCs were cocultured with retinal cells, with or without 
      Fc-TrK inhibitors, in a Transwell system, and the number of surviving 
      betaIII-tubulin(+) retinal cells and length/number of betaIII-tubulin(+) neurites were 
      quantified. For the in vivo study, DPSCs or BMSCs were transplanted into the 
      vitreous body of the eye after a surgically induced optic nerve crush injury. At 
      7, 14, and 21 days postlesion (dpl), optical coherence tomography (OCT) was used 
      to measure the retinal nerve fiber layer thickness as a measure of axonal 
      atrophy. At 21 dpl, numbers of Brn-3a(+) RGCs in parasagittal retinal sections and 
      growth-associated protein-43(+) axons in longitudinal optic nerve sections were 
      quantified as measures of RGC survival and axon regeneration, respectively. 
      RESULTS: Both DPSCs and BMSCs secreted NGF, BDNF, and NT-3, with DPSCs secreting 
      significantly higher titers of NGF and BDNF than BMSCs. DPSCs, and to a lesser 
      extent BMSCs, promoted statistically significant survival and 
      neuritogenesis/axogenesis of betaIII-tubulin(+) retinal cells in vitro and in vivo 
      where the effects were abolished after TrK receptor blockade. CONCLUSIONS: 
      Intravitreal transplants of DPSCs promoted significant neurotrophin-mediated RGC 
      survival and axon regeneration after optic nerve injury.
FAU - Mead, Ben
AU  - Mead B
AD  - Neurotrauma and Neurodegeneration Section, School of Clinical and Experimental 
      Medicine, University of Birmingham, Birmingham, United Kingdom.
FAU - Logan, Ann
AU  - Logan A
FAU - Berry, Martin
AU  - Berry M
FAU - Leadbeater, Wendy
AU  - Leadbeater W
FAU - Scheven, Ben A
AU  - Scheven BA
LA  - eng
GR  - BB/F017553/1/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131115
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Axons/*physiology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Coculture Techniques
MH  - Dental Pulp/*cytology/metabolism
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Nerve Growth Factor/metabolism
MH  - Nerve Regeneration/*physiology
MH  - Neurotrophin 3/metabolism
MH  - Optic Nerve Injuries/metabolism/*prevention & control
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinal Ganglion Cells/*physiology
MH  - *Stem Cell Transplantation
MH  - Stem Cells/physiology
MH  - Tomography, Optical Coherence
MH  - Vitreous Body/*surgery
OTO - NOTNLM
OT  - axon regeneration
OT  - cell transplantation
OT  - dental pulp stem cells
OT  - mesenchymal stem cells
OT  - neuroprotection
EDAT- 2013/10/24 06:00
MHDA- 2014/01/25 06:00
CRDT- 2013/10/24 06:00
PHST- 2013/10/24 06:00 [entrez]
PHST- 2013/10/24 06:00 [pubmed]
PHST- 2014/01/25 06:00 [medline]
AID - iovs.13-13045 [pii]
AID - 10.1167/iovs.13-13045 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2013 Nov 15;54(12):7544-56. doi: 
      10.1167/iovs.13-13045.