PMID- 24151629
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2013
DP  - 2013
TI  - Anti-inflammatory activity of bioaccessible fraction from Eryngium foetidum 
      leaves.
PG  - 958567
LID - 10.1155/2013/958567 [doi]
LID - 958567
AB  - Eryngium foetidum (EF) has long been used as a medicinal plant and culinary spice 
      in tropical regions. Phytochemicals in its leaves have been proposed to be 
      responsible for the anti-inflammatory and antioxidant activities. The present 
      study used in vitro digestion coupled with Caco-2 cells to assess such 
      activities. Caco-2 cells were incubated with aqueous fraction from simulated 
      digestion (bioaccessible fraction) of EF leaves with/without bile extract prior 
      to stimulation with interleukin-1 beta (IL-1beta). Monocyte chemoattractant 
      protein-1 (MCP-1) and IL-8 in culture media and the intracellular reactive oxygen 
      species (ROS) were measured. Approximately 24% beta-carotene and 35% lutein of 
      leaves were present in the aqueous fraction. The transfer of caffeic and 
      chlorogenic acids to the aqueous fraction was 76%-81%, while that of kaempferol 
      was 48%. Prior incubation of Caco-2 cells with the bioaccessible fraction 
      suppressed IL-1beta activated IL-8 and MCP-1 by 33%, but the fraction lacking mixed 
      micelles decreased IL-8 and MCP-1 levels only by 11%. The pretreatment of Caco-2 
      cells with the bioaccessible fraction of EF reduced ROS by 34%; the fraction 
      lacking mixed micelles decreased ROS by 28%. These data suggest that bioactive 
      compounds partitioning in mixed micelles play a significant role to suppress the 
      proinflammatory insult but with a modest antioxidant effect.
FAU - Dawilai, Suwitcha
AU  - Dawilai S
AD  - Institute of Nutrition, Mahidol University, Salaya, Nakhon Pathom 73170, 
      Thailand.
FAU - Muangnoi, Chawanphat
AU  - Muangnoi C
FAU - Praengamthanachoti, Phawachaya
AU  - Praengamthanachoti P
FAU - Tuntipopipat, Siriporn
AU  - Tuntipopipat S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130917
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-8)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 01YAE03M7J (beta Carotene)
RN  - X72A60C9MT (Lutein)
SB  - IM
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Antioxidants/pharmacology
MH  - Caco-2 Cells
MH  - Chemokine CCL2/biosynthesis
MH  - Eryngium/*chemistry
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Inflammation/*drug therapy/pathology
MH  - Interleukin-1beta/biosynthesis
MH  - Interleukin-8/biosynthesis
MH  - Lutein/chemistry/isolation & purification
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Plant Leaves/chemistry
MH  - Reactive Oxygen Species/metabolism
MH  - beta Carotene/chemistry/isolation & purification
PMC - PMC3789289
EDAT- 2013/10/24 06:00
MHDA- 2014/06/03 06:00
PMCR- 2013/09/17
CRDT- 2013/10/24 06:00
PHST- 2013/04/30 00:00 [received]
PHST- 2013/07/29 00:00 [revised]
PHST- 2013/08/01 00:00 [accepted]
PHST- 2013/10/24 06:00 [entrez]
PHST- 2013/10/24 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
PHST- 2013/09/17 00:00 [pmc-release]
AID - 10.1155/2013/958567 [doi]
PST - ppublish
SO  - Biomed Res Int. 2013;2013:958567. doi: 10.1155/2013/958567. Epub 2013 Sep 17.