PMID- 24152676
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20211021
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 18
IP  - 10
DP  - 2013 Oct 21
TI  - Sesquiterpene lactones and their derivatives inhibit high glucose-induced NF-kappaB 
      activation and MCP-1 and TGF-beta1 expression in rat mesangial cells.
PG  - 13061-77
LID - 10.3390/molecules181013061 [doi]
AB  - Diabetic nephropathy (DN) is one of the most common and serious chronic 
      complications of diabetes mellitus, however, no efficient clinical drugs exist 
      for the treatment of DN. We selected and synthesized several sesquiterpene 
      lactones (SLs), and then used the MTT assay to detect rat mesangial cells (MCs) 
      proliferation, ELISA to measure the expression level of monocyte chemoattractant 
      protein-1 (MCP-1), transforming growth factor beta (TGF-beta1) and fibronectin(FN), 
      real-time fluorescent quantitative PCR analysis to measure the MCP-1 and TGF-beta1 
      gene expression, western blot to detect the level of IkappaBalpha protein and EMSA to 
      measure the activation of nuclear factor kappa B (NF-kappaB). We discovered that SLs, 
      including parthenolide (PTL), micheliolide (MCL), arglabin, and isoalantolactone 
      (IAL), as well as several synthetic analogs of these molecules, could effectively 
      attenuate the high glucose-stimulated activation of NF-kappaB, the degradation of 
      IkappaBalpha, and the expression of MCP-1, TGF-beta1 and FN in rat mesangial cells (MCs). 
      These findings suggest that SLs and their derivatives have potential as candidate 
      drugs for the treatment of DN.
FAU - Jia, Qian-Qian
AU  - Jia QQ
AD  - Department of Nephrology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou 510000, China. zjyy@smu.edu.cn.
FAU - Wang, Jian-Cheng
AU  - Wang JC
FAU - Long, Jing
AU  - Long J
FAU - Zhao, Yan
AU  - Zhao Y
FAU - Chen, Si-Jia
AU  - Chen SJ
FAU - Zhai, Jia-Dai
AU  - Zhai JD
FAU - Wei, Lian-Bo
AU  - Wei LB
FAU - Zhang, Quan
AU  - Zhang Q
FAU - Chen, Yue
AU  - Chen Y
FAU - Long, Hai-Bo
AU  - Long HB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131021
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lactones)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sesquiterpenes)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta1)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Diabetic Nephropathies/drug therapy
MH  - Drug Evaluation, Preclinical
MH  - Gene Expression/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Glucose/*physiology
MH  - Lactones/chemical synthesis/*pharmacology
MH  - Mesangial Cells/drug effects/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Sesquiterpenes/chemical synthesis/*pharmacology
MH  - Transforming Growth Factor beta1/genetics/*metabolism
PMC - PMC6269856
EDAT- 2013/10/25 06:00
MHDA- 2014/04/23 06:00
PMCR- 2013/10/21
CRDT- 2013/10/25 06:00
PHST- 2013/09/12 00:00 [received]
PHST- 2013/09/12 00:00 [revised]
PHST- 2013/10/14 00:00 [accepted]
PHST- 2013/10/25 06:00 [entrez]
PHST- 2013/10/25 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
PHST- 2013/10/21 00:00 [pmc-release]
AID - molecules181013061 [pii]
AID - molecules-18-13061 [pii]
AID - 10.3390/molecules181013061 [doi]
PST - epublish
SO  - Molecules. 2013 Oct 21;18(10):13061-77. doi: 10.3390/molecules181013061.