PMID- 24155319 OWN - NLM STAT- MEDLINE DCOM- 20131223 LR - 20220309 IS - 1529-2401 (Electronic) IS - 0270-6474 (Print) IS - 0270-6474 (Linking) VI - 33 IP - 43 DP - 2013 Oct 23 TI - Dynamic and permissive roles of TRPV1 and TRPV4 channels for thermosensation in mouse supraoptic magnocellular neurosecretory neurons. PG - 17160-5 LID - 10.1523/JNEUROSCI.1048-13.2013 [doi] AB - The transient receptor potential vanilloid 1 and 4 genes (trpv1, trpv4) encode temperature-sensitive cation channels hypothesized to mediate thermoresponses in mammalian cells. Although such channels were shown to participate in the peripheral detection of ambient temperature, the specific roles of these channels in central thermosensory neurons remain unclear. Here we report that the membrane potential and excitability of mouse magnocellular neurosecretory cells (MNCs) maintained at physiological temperature were lowered in an additive manner upon pharmacological blockade, or genetic deletion, of trpv1 and trpv4. However extracellular recordings from spontaneously active MNCs in situ showed that blockade or genetic deletion of trpv4 does not interfere with thermally induced changes in action potential firing, whereas loss of trpv1 abolished this phenotype. These findings indicate that channels encoded by trpv4 play a permissive role that contributes to basal electrical activity, but that trpv1 plays a dynamic role that is required for physiological thermosensation by MNCs. FAU - Sudbury, Jessica R AU - Sudbury JR AD - Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada. FAU - Bourque, Charles W AU - Bourque CW LA - eng GR - MOP-82818/Canadian Institutes of Health Research/Canada GR - MOP-9939/Canadian Institutes of Health Research/Canada PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 RN - 0 (TRPV Cation Channels) RN - 0 (TRPV1 protein, mouse) RN - 0 (Trpv4 protein, mouse) SB - IM MH - Action Potentials MH - Animals MH - Hypothalamus, Anterior/cytology/metabolism/*physiology MH - Membrane Potentials MH - Mice MH - Mutation MH - Neurons/metabolism/*physiology MH - Phenotype MH - TRPV Cation Channels/genetics/*metabolism MH - *Thermosensing PMC - PMC6618445 EDAT- 2013/10/25 06:00 MHDA- 2013/12/24 06:00 PMCR- 2014/04/23 CRDT- 2013/10/25 06:00 PHST- 2013/10/25 06:00 [entrez] PHST- 2013/10/25 06:00 [pubmed] PHST- 2013/12/24 06:00 [medline] PHST- 2014/04/23 00:00 [pmc-release] AID - 33/43/17160 [pii] AID - 1048-13 [pii] AID - 10.1523/JNEUROSCI.1048-13.2013 [doi] PST - ppublish SO - J Neurosci. 2013 Oct 23;33(43):17160-5. doi: 10.1523/JNEUROSCI.1048-13.2013.