PMID- 24156086
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131024
LR  - 20211021
IS  - 2193-1801 (Print)
IS  - 2193-1801 (Electronic)
IS  - 2193-1801 (Linking)
VI  - 2
DP  - 2013
TI  - Status of the anaplastic lymphoma kinase (ALK) gene in inflammatory breast 
      carcinoma.
PG  - 409
LID - 10.1186/2193-1801-2-409 [doi]
LID - 409
AB  - BACKGROUND: Although preliminary reports suggest that ALK gene amplification may 
      occur in inflammatory breast cancer (IBC), data are limited. We performed a 
      comprehensive investigation of the status of ALK gene in IBC. METHODS: We used 
      core biopsy (CB) samples from 30 IBC patients for immunohistochemistry (IHC), 25 
      of these samples for fluorescence in situ hybridization (FISH) of ALK gene 
      rearrangement, 8 for chromosome 2 aneusomy, and 20 microdissected frozen CBs for 
      array comparative genomic hybridization (CGH) and mRNA analysis. RESULTS: All 30 
      samples were negative for ALK protein expression by IHC. FISH analysis showed no 
      EML4-ALK gene rearrangement in any samples, although 16 of the 25 samples (64%) 
      contained 3 to 4 extra copies of the ALK gene, and chromosome 2 aneusomy was 
      found in 7 of 8 samples that had extra copies of the ALK gene. Only 3 of the 20 
      samples showed evidence of mild ALK gene amplification by array CGH. mRNA 
      analysis revealed that mRNA expression of ALK was not significantly higher in 
      these samples compared with samples that showed no evidence of ALK gene 
      amplification in CGH analysis, nor was mRNA expression of ALK significantly 
      different in tumor compared with 5 normal breast samples (P > 0.05, t test). 
      CONCLUSION: Our comprehensive evaluation suggests that ALK gene rearrangement did 
      not occur in the IBC patients studied. The significance of our finding of mildly 
      increased copy numbers of the ALK gene resulting from chromosome 2 aneusomy 
      rather than mild amplification of the ALK gene requires further investigation.
FAU - Krishnamurthy, Savitri
AU  - Krishnamurthy S
AD  - Department of Pathology, The University of Texas MD Anderson Cancer Center, 1515 
      Holcombe Blvd., Unit 53, Houston, TX 77030 USA.
FAU - Woodward, Wendy
AU  - Woodward W
FAU - Yang, Wei
AU  - Yang W
FAU - Reuben, James M
AU  - Reuben JM
FAU - Tepperberg, James
AU  - Tepperberg J
FAU - Ogura, Dai
AU  - Ogura D
FAU - Niwa, Shin-Ichiro
AU  - Niwa S
FAU - Huo, Lei
AU  - Huo L
FAU - Gong, Yun
AU  - Gong Y
FAU - El-Zein, Randa
AU  - El-Zein R
FAU - Gonzalez-Angulo, Ana M
AU  - Gonzalez-Angulo AM
FAU - Chavez-Macgregor, Mariana
AU  - Chavez-Macgregor M
FAU - Alvarez, Ricardo
AU  - Alvarez R
FAU - Lucci, Anthony
AU  - Lucci A
FAU - Valero, Vicente
AU  - Valero V
FAU - Ueno, Naoto T
AU  - Ueno NT
LA  - eng
PT  - Journal Article
DEP - 20130828
PL  - Switzerland
TA  - Springerplus
JT  - SpringerPlus
JID - 101597967
PMC - PMC3797914
EDAT- 2013/10/25 06:00
MHDA- 2013/10/25 06:01
PMCR- 2013/08/28
CRDT- 2013/10/25 06:00
PHST- 2013/08/21 00:00 [received]
PHST- 2013/08/23 00:00 [accepted]
PHST- 2013/10/25 06:00 [entrez]
PHST- 2013/10/25 06:00 [pubmed]
PHST- 2013/10/25 06:01 [medline]
PHST- 2013/08/28 00:00 [pmc-release]
AID - 583 [pii]
AID - 10.1186/2193-1801-2-409 [doi]
PST - epublish
SO  - Springerplus. 2013 Aug 28;2:409. doi: 10.1186/2193-1801-2-409. eCollection 2013.