PMID- 24156451
OWN - NLM
STAT- MEDLINE
DCOM- 20140505
LR  - 20211203
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 21
IP  - 5
DP  - 2013 Oct
TI  - [Effects of N-Arachidonoylethanolamine on the quality of platelets stored in 
      M-sol platelet preservative solution in vitro].
PG  - 1285-90
LID - 10.7534/j.issn.1009-2137.2013.05.040 [doi]
AB  - This study was purposed to investigate the effects of N-Arachidonoylethanolamine 
      (ANA) on the quality of platelets (Plt) stored in Plt M-sol preservative solution 
      at 22 +/- 2 degrees C. Samples taken from collecting apheresis Plt by the Amicus instrument 
      and splited into two equal parts were stored in Plt M-sol preservative solution 
      on a shaker at 22 +/- 2 degrees C. Different working concentrations of ANA (from 0.1 to 50 
      micromol/L) were then added into one part of stored Plt as the experimental group, 
      the other without ANA was used as the control group. The viability of Plts stored 
      at 22 +/- 2 degrees C for 7 days was evaluated by MTT colorimetric assay. The most 
      effective concentration of ANA was selected and added to the subsequent 
      experimental group. Plt count (BPC), mean Plt volume (MPV), Plt distribution 
      width (PDW), phosphatidyl serine (PS) and soluble P-selectin were detected on the 
      1(st), 5(th), 7(th), 9(th) and 11(th) day of storage. The results showed that the 
      most effective working concentration of ANA was 0.5 micromol/L, which showed 
      significant increasing Plt viability (91.23 +/- 5.44%) compared to the control 
      group (62.54 +/- 4.79%). Thus, ANA concentration at 0.5 micromol/L was choose to 
      perform subsequent experiments. During 11 days of storage, the BPC, MPV and PDW 
      were not changed significantly between the experimental group and control group, 
      although there was decreasing trend in the BPC and increasing trends in MPV and 
      PDW in the two groups. The rate of Plt PS positive was enhanced during the 
      storage period: the rate of PS positive in experimental group increased from 7.69 
      +/- 1.82% to 10.74 +/- 1.78% while it in control group increased from 11.21 +/- 2.03% 
      to 15.37 +/- 1.95%, with significant differences between the two groups (P < 0.05) 
      on the 9(th) and 11(th) day of storage, respectively. Soluble P-selectin contents 
      in experimental group on the 9(th) and 11(th) day of storage were 30.19 +/- 2.03 
      ng/ml and 34.52 +/- 2.64 ng/mL, respectively, while those in control group were 
      39.18 +/- 2.66 ng/ml and 43.23 +/- 2.58 ng/ml, respectively, with significant 
      differences between the two groups (P < 0.05). It is concluded that the extended 
      storage of Plt in M-sol treated with low concentration ANA can potentially 
      alleviate Plt storage lesions.
FAU - Zhuang, Yun-Long
AU  - Zhuang YL
AD  - Blood Center of Shandong Province, Jinan 250014, Shandong Province, China. 
      E-mail:yunlongzhuang@gmail.com.
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Qiao, Wen-Ben
AU  - Qiao WB
FAU - Yu, Yuan
AU  - Yu Y
FAU - Lin, Ming
AU  - Lin M
FAU - Zhu, Qing
AU  - Zhu Q
FAU - Zhou, Juan
AU  - Zhou J
FAU - Sun, Gui-Zhi
AU  - Sun GZ
FAU - Zhao, Cui-Yun
AU  - Zhao CY
FAU - Nie, Xiang-Min
AU  - Nie XM
FAU - Liu, Hong
AU  - Liu H
FAU - Chen, Yuan-Feng
AU  - Chen YF
FAU - Zhu, Chuan-Fu
AU  - Zhu CF
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Arachidonic Acids)
RN  - 0 (Endocannabinoids)
RN  - 0 (Polyunsaturated Alkamides)
RN  - UR5G69TJKH (anandamide)
SB  - IM
MH  - Adult
MH  - Arachidonic Acids
MH  - Blood Platelets/*drug effects
MH  - *Blood Preservation
MH  - Endocannabinoids/*pharmacology
MH  - Female
MH  - Humans
MH  - Male
MH  - Polyunsaturated Alkamides
EDAT- 2013/10/26 06:00
MHDA- 2014/05/06 06:00
CRDT- 2013/10/26 06:00
PHST- 2013/10/26 06:00 [entrez]
PHST- 2013/10/26 06:00 [pubmed]
PHST- 2014/05/06 06:00 [medline]
AID - 1009-2137(2013)05-1285-06 [pii]
AID - 10.7534/j.issn.1009-2137.2013.05.040 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Oct;21(5):1285-90. doi: 
      10.7534/j.issn.1009-2137.2013.05.040.