PMID- 24156528 OWN - NLM STAT- MEDLINE DCOM- 20140902 LR - 20131127 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 61 IP - 47 DP - 2013 Nov 27 TI - Investigating the molecular structural features of hulless barley (Hordeum vulgare L.) in relation to metabolic characteristics using synchrotron-based fourier transform infrared microspectroscopy. PG - 11250-60 LID - 10.1021/jf403196z [doi] AB - The synchrotron-based Fourier transform infrared microspectroscopy (SR-FTIRM) technique was used to quantify molecular structural features of the four hulless barley lines with altered carbohydrate traits [amylose, 1-40% of dry matter (DM); beta-glucan, 5-10% of DM] in relation to rumen degradation kinetics, intestinal nutrient digestion, and predicted protein supply. Spectral features of beta-glucan (both area and heights) in hulless barley lines showed a negative correlation with protein availability in the small intestine, including truly digested protein in the small intestine (DVE) (r = -0.76, P < 0.01; r = -0.84, P < 0.01) and total metabolizable protein (MP) (r = -0.71, P < 0.05; r = -0.84, P < 0.01). Variation in absorption intensities of total carbohydrate (CHO) was observed with negative effects on protein degradation, digestion, and potential protein supply (P < 0.05). Molecular structural features of CHO in hulless barley have negative effects on the supply of true protein to ruminants. The results clearly indicated the impact of the carbohydrate-protein structure and matrix. FAU - Yang, Ling AU - Yang L AD - Department of Animal and Poultry Science and double daggerCrop Development Centre, College of Agriculture and Bioresources, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada. FAU - Christensen, David A AU - Christensen DA FAU - McKinnon, John J AU - McKinnon JJ FAU - Beattie, Aaron D AU - Beattie AD FAU - Xin, Hangshu AU - Xin H FAU - Yu, Peiqiang AU - Yu P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131118 PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Carbohydrates) RN - 0 (Plant Proteins) RN - 0 (beta-Glucans) RN - 9005-82-7 (Amylose) SB - IM MH - Amylose/metabolism MH - Animal Feed MH - Animals MH - Carbohydrates/chemistry MH - Endosperm/chemistry MH - Hordeum/*chemistry/*metabolism MH - Kinetics MH - Plant Proteins/chemistry/pharmacokinetics MH - Rumen/metabolism MH - Ruminants/metabolism MH - Spectroscopy, Fourier Transform Infrared/*methods MH - Synchrotrons MH - beta-Glucans/metabolism EDAT- 2013/10/26 06:00 MHDA- 2014/09/03 06:00 CRDT- 2013/10/26 06:00 PHST- 2013/10/26 06:00 [entrez] PHST- 2013/10/26 06:00 [pubmed] PHST- 2014/09/03 06:00 [medline] AID - 10.1021/jf403196z [doi] PST - ppublish SO - J Agric Food Chem. 2013 Nov 27;61(47):11250-60. doi: 10.1021/jf403196z. Epub 2013 Nov 18.