PMID- 24157330
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20131206
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 17
IP  - 4
DP  - 2013 Dec
TI  - Curcumin inhibits agent-induced human neutrophil functions in vitro and 
      lipopolysaccharide-induced neutrophilic infiltration in vivo.
PG  - 1101-7
LID - S1567-5769(13)00373-1 [pii]
LID - 10.1016/j.intimp.2013.09.024 [doi]
AB  - Curcumin, extracted from the rhizome of Curcuma longa, is known to possess 
      anti-inflammatory activities. Despite the fact that neutrophils are key player 
      cells in inflammation, the role of curcumin on neutrophil cell biology is not 
      well documented and, in particular, how curcumin can alter primed neutrophils is 
      unknown. In addition, the effect of curcumin on agent-induced neutrophilic 
      inflammation is not well documented. Here, we demonstrated that curcumin 
      inhibited formyl-methionyl-leucyl-phenylalanine (fMLP)- or lipopolysaccharide 
      (LPS)-induced suppression of human neutrophil apoptosis. In addition, we found 
      that curcumin reversed the ability of phorbol myristate acetate (PMA) to induce 
      reactive oxygen species as assessed by flow cytometry using the CM-H2DCF-DA 
      probe. Using an antibody array approach, curcumin was found to inhibit 
      LPS-induced cytokine production, including MIP-1alpha, MIP-1beta, IL-6, IL-8 (CXCL-8) 
      and GRO-alpha. The inhibitory effect of curcumin on IL-8 production was confirmed by 
      ELISA. Using both an electrophoretic mobility shift assay and a TransFactor p50 
      NF-kappaB ELISA, we demonstrated that curcumin inhibited LPS-induced NF-kappaB 
      activation. In vivo, using the murine air pouch model of acute inflammation, we 
      demonstrated that intraperitoneal administration of curcumin inhibited 
      LPS-induced neutrophilic infiltration in vivo. As assessed by a murine antibody 
      array approach, curcumin was found to decrease the local production of several 
      cytokines/chemokines induced by LPS, including, but not limit to, MIP-1alpha and 
      MIP-1beta. We conclude that curcumin possesses potent modulatory activities on 
      primed or agent-induced human neutrophils in vitro and that it possesses 
      important anti-inflammatory activities in vivo by inhibiting LPS-induced 
      neutrophilic inflammation.
CI  - (c) 2013.
FAU - Antoine, Francis
AU  - Antoine F
AD  - Laboratoire de recherche en inflammation et physiologie des granulocytes, 
      Universite du Quebec, INRS-Institut-Armand-Frappier, Laval, Quebec Canada.
FAU - Simard, Jean-Christophe
AU  - Simard JC
FAU - Girard, Denis
AU  - Girard D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131021
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cells, Cultured
MH  - Curcumin/*pharmacology
MH  - Cytokines/metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/metabolism
MH  - Interleukin-8/metabolism
MH  - Lipopolysaccharides
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Neutrophils/*drug effects/metabolism
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Curcumin
OT  - Immunomodulation
OT  - Inflammation
OT  - Neutrophils
EDAT- 2013/10/26 06:00
MHDA- 2014/09/03 06:00
CRDT- 2013/10/26 06:00
PHST- 2013/08/14 00:00 [received]
PHST- 2013/09/18 00:00 [revised]
PHST- 2013/09/30 00:00 [accepted]
PHST- 2013/10/26 06:00 [entrez]
PHST- 2013/10/26 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
AID - S1567-5769(13)00373-1 [pii]
AID - 10.1016/j.intimp.2013.09.024 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2013 Dec;17(4):1101-7. doi: 10.1016/j.intimp.2013.09.024. 
      Epub 2013 Oct 21.