PMID- 24157978
OWN - NLM
STAT- MEDLINE
DCOM- 20140815
LR  - 20141120
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 720
IP  - 1-3
DP  - 2013 Nov 15
TI  - Involvement of IP3-receptor activation in endothelin-1-induced Ca(2+) influx in 
      rat pulmonary small artery.
PG  - 255-63
LID - S0014-2999(13)00774-7 [pii]
LID - 10.1016/j.ejphar.2013.09.076 [doi]
AB  - We examined the endothelin-1 (ET-1)-induced increase in the intracellular free 
      Ca(2+) concentration ([Ca(2+)]i) in fura-2-loaded rat pulmonary small arteries. 
      ET-1 (30 nM) elicited a long-lasting increase in [Ca(2+)]i in physiological salt 
      solution (PSS). In subsequent experiments, arteries were pretreated with BQ-788, 
      an ETB-specific blocker, to allow us to focus on responses mediated via the ETA 
      receptor, the existence of which was confirmed by immunohistochemistry. In 
      Ca(2+)-free PSS, ET-1 evoked a small transient increase in [Ca(2+)]i, indicating 
      Ca(2+) release from the SR (sarcoplasmic reticulum). After a switch to PSS 
      (containing 2mM CaCl2), ET-1 elicited a long-lasting increase in [Ca(2+)]i that 
      was not inhibited by 1 muM nicardipine, an L-type Ca(2+)-channel inhibitor, 
      suggesting involvement of a Ca(2+)-influx pathway independent of that channel. In 
      arteries preincubated with 30 muM cyclopiazonic acid (CPA) or 2 muM thapsigargin 
      (TG), the ET-1-induced Ca(2+)-release was greatly reduced, and the induced 
      Ca(2+)-influx was attenuated. U-73122, a phospholipase C (PLC) inhibitor, had 
      inhibitory effects similar to those of CPA and TG on the ET-1-induced 
      Ca(2+)-release and Ca(2+)-influx, whereas U-73343, an inactive analogue of 
      U-73122, had no such effects. Two putative membrane-permeable IP3-receptor 
      blockers, 2-aminoethoxydiphenyl borate (2APB, 50 muM) and Xestospongin C (20 muM), 
      (a) almost completely inhibited the ET-1-induced Ca(2+)-release and 
      Ca(2+)-influx, and (b) reduced the ET-1-induced contraction. These results 
      indicate that in rat pulmonary small arteries, ET-1 induces receptor-operated 
      Ca(2+) influx via the ETA receptor, and that this influx interacts with 
      InsP3-receptor activation.
CI  - (c) 2013 Published by Elsevier B.V.
FAU - Kato, K
AU  - Kato K
AD  - Department of Physiological Science and Molecular Biology, Fukuoka Dental 
      College, Fukuoka, Japan; Fukuoka School of Medical Sciences, Fukuoka, Japan. 
      Electronic address: kkatou@college.fdcnet.ac.jp.
FAU - Okamura, K
AU  - Okamura K
FAU - Hatta, M
AU  - Hatta M
FAU - Morita, H
AU  - Morita H
FAU - Kajioka, S
AU  - Kajioka S
FAU - Naito, S
AU  - Naito S
FAU - Yamazaki, J
AU  - Yamazaki J
LA  - eng
PT  - Journal Article
DEP - 20131022
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Endothelin-1)
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 0 (Receptor, Endothelin A)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Endothelin-1/*metabolism
MH  - In Vitro Techniques
MH  - Inositol 1,4,5-Trisphosphate Receptors/*metabolism
MH  - Male
MH  - Pulmonary Artery/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Endothelin A/metabolism
OTO - NOTNLM
OT  - 2APB
OT  - Fura-2
OT  - IP3
OT  - Pulmonary artery
OT  - ROCC
OT  - Xestospongin C
EDAT- 2013/10/26 06:00
MHDA- 2014/08/16 06:00
CRDT- 2013/10/26 06:00
PHST- 2013/04/03 00:00 [received]
PHST- 2013/07/25 00:00 [revised]
PHST- 2013/09/13 00:00 [accepted]
PHST- 2013/10/26 06:00 [entrez]
PHST- 2013/10/26 06:00 [pubmed]
PHST- 2014/08/16 06:00 [medline]
AID - S0014-2999(13)00774-7 [pii]
AID - 10.1016/j.ejphar.2013.09.076 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2013 Nov 15;720(1-3):255-63. doi: 10.1016/j.ejphar.2013.09.076. 
      Epub 2013 Oct 22.