PMID- 24160183
OWN - NLM
STAT- MEDLINE
DCOM- 20150406
LR  - 20211021
IS  - 1751-0147 (Electronic)
IS  - 0044-605X (Print)
IS  - 0044-605X (Linking)
VI  - 55
IP  - 1
DP  - 2013 Oct 25
TI  - The use of the rapid osmotic fragility test as an additional test to diagnose 
      canine immune-mediated haemolytic anaemia.
PG  - 74
LID - 10.1186/1751-0147-55-74 [doi]
AB  - BACKGROUND: Diagnosing canine immune-mediated haemolytic anaemia (IMHA) is often 
      challenging because all currently available tests have their limitations. Dogs 
      with IMHA often have an increased erythrocyte osmotic fragility (OF), a 
      characteristic that is sometimes used in the diagnosis of IMHA. Since the classic 
      osmotic fragility test (COFT) is time-consuming and requires specialized 
      equipment, an easy and less labour-intensive rapid osmotic fragility test (ROFT) 
      has been used in some countries, but its diagnostic value has not yet been 
      investigated.This study aimed to evaluate erythrocyte osmotic fragility in dogs 
      with and without IMHA, to compare results of the classic (COFT) and rapid (ROFT) 
      test and to assess the value of the ROFT as diagnostic test for canine 
      IMHA.Nineteen dogs with IMHA (group 1a), 21 anaemic dogs without IMHA (group 1b), 
      8 dogs with microcytosis (group 2), 13 hyperlipemic dogs (group 3), 10 dogs with 
      lymphoma (group 4), 8 dogs with an infection (group 5) and 13 healthy dogs (group 
      6) were included.In all dogs, blood smear examination, in-saline 
      auto-agglutination test, Coombs' test, COFT and ROFT were performed. In the COFT, 
      OF5, OF50 and OF90 were defined as the NaCl concentrations at which respectively 
      5, 50 and 90% of erythrocytes were haemolysed. RESULTS: Compared with healthy 
      dogs, OF5 and OF50 were significantly higher in group 1a (P < 0.001) and OF5 was 
      significantly higher in group 3 (P = 0.0266). The ROFT was positive in 17 dogs 
      with IMHA, 10 hyperlipemic dogs, one anaemic dog without IMHA and one healthy 
      dog. CONCLUSIONS: Osmotic fragility was increased in the majority of dogs with 
      IMHA and in dogs with hyperlipidemia, but not in dogs with microcytosis, lymphoma 
      or an infection. Although more detailed information was obtained about the 
      osmotic fragility by using the COFT, the COFT and ROFT gave similar results. The 
      ROFT does not require specialized equipment, is rapid and easy to perform and can 
      be used easily in daily practice. Although, the ROFT cannot replace other 
      diagnostic tests, it may be a valuable additional tool to diagnose canine IMHA.
FAU - Paes, Geert
AU  - Paes G
AD  - Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary 
      Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium. 
      geertpaes@gmail.com.
FAU - Paepe, Dominique
AU  - Paepe D
FAU - Meyer, Evelyne
AU  - Meyer E
FAU - Kristensen, Annemarie T
AU  - Kristensen AT
FAU - Duchateau, Luc
AU  - Duchateau L
FAU - Campos, Miguel
AU  - Campos M
FAU - Daminet, Sylvie
AU  - Daminet S
LA  - eng
PT  - Journal Article
DEP - 20131025
PL  - England
TA  - Acta Vet Scand
JT  - Acta veterinaria Scandinavica
JID - 0370400
SB  - IM
MH  - Anemia, Hemolytic, Autoimmune/blood/diagnosis/*veterinary
MH  - Animals
MH  - Dog Diseases/blood/*diagnosis
MH  - Dogs
MH  - Osmotic Fragility/*physiology
MH  - Predictive Value of Tests
MH  - Prospective Studies
PMC - PMC3816578
EDAT- 2013/10/29 06:00
MHDA- 2015/04/07 06:00
PMCR- 2013/10/25
CRDT- 2013/10/29 06:00
PHST- 2013/01/28 00:00 [received]
PHST- 2013/10/21 00:00 [accepted]
PHST- 2013/10/29 06:00 [entrez]
PHST- 2013/10/29 06:00 [pubmed]
PHST- 2015/04/07 06:00 [medline]
PHST- 2013/10/25 00:00 [pmc-release]
AID - 1751-0147-55-74 [pii]
AID - 10.1186/1751-0147-55-74 [doi]
PST - epublish
SO  - Acta Vet Scand. 2013 Oct 25;55(1):74. doi: 10.1186/1751-0147-55-74.