PMID- 24161393
OWN - NLM
STAT- MEDLINE
DCOM- 20140117
LR  - 20131118
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 441
IP  - 2
DP  - 2013 Nov 15
TI  - Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the 
      pro-inflammatory effects of caveolin-1 in endothelial cells.
PG  - 405-10
LID - S0006-291X(13)01755-5 [pii]
LID - 10.1016/j.bbrc.2013.10.074 [doi]
AB  - Pigment epithelium-derived factor (PEDF) exerts atheroprotective effects both in 
      cell culture and animal models through its anti-oxidative and anti-inflammatory 
      properties. Caveolin-1 (Cav), a major protein component of caveolae in 
      endothelial cells (ECs), plays a role in the progression of atherosclerosis. 
      However, effects of PEDF on Cav-exposed ECs remain unknown. In this study, we 
      examined whether and how PEDF could inhibit the Cav-induced inflammatory and 
      thrombogenic reactions in human umbilical vein ECs (HUVECs). Surface plasmon 
      resonance revealed that PEDF bound to Cav at the dissociation constant of 
      7.36x10(-7) M. Further, one of the major Cav-interacting proteins in human serum 
      was identified as PEDF by peptide mass fingerprinting analysis using BIAcore 1000 
      combined with matrix-assisted laser desorption/ionization time-of-flight mass 
      spectrometry. Exogenously added Cav was taken up into the membrane fraction of 
      HUVECs and dose-dependently increased monocyte chemoattractant protein-1 (MCP-1), 
      vascular cell adhesion molecule-1 (VCAM-1) and plasminogen activator inhibitor-1 
      (PAI-1) mRNA levels, all of which were blocked by the simultaneous treatment with 
      10nM PEDF. Small interfering RNAs directed against Cav decreased endogenous Cav 
      levels and suppressed gene expression of MCP-1, VCAM-1 and PAI-1 in HUVECs. This 
      study indicates that PEDF binds to Cav and could block the inflammatory and 
      thrombogenic reactions in Cav-exposed HUVECs. Our present study suggests that 
      atheroprotective effects of PEDF might be partly ascribed to its Cav-interacting 
      properties.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Matsui, Takanori
AU  - Matsui T
AD  - Department of Pathophysiology and Therapeutics of Diabetic Vascular 
      Complications, Kurume University School of Medicine, Kurume, Japan.
FAU - Higashimoto, Yuichiro
AU  - Higashimoto Y
FAU - Taira, Junichi
AU  - Taira J
FAU - Yamagishi, Sho-ichi
AU  - Yamagishi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131023
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CAV1 protein, human)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Caveolin 1)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Eye Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Plasminogen Activator Inhibitor 1)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Serpins)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (pigment epithelium-derived factor)
SB  - IM
MH  - Atherosclerosis/genetics/*metabolism
MH  - Caveolin 1/genetics/*metabolism/pharmacology
MH  - Chemokine CCL2/genetics
MH  - Eye Proteins/genetics/*metabolism/pharmacology
MH  - Gene Expression/drug effects
MH  - Human Umbilical Vein Endothelial Cells/*metabolism
MH  - Humans
MH  - Inflammation/genetics/*metabolism
MH  - Nerve Growth Factors/genetics/*metabolism/pharmacology
MH  - Plasminogen Activator Inhibitor 1/genetics
MH  - RNA, Small Interfering/genetics
MH  - Serpins/genetics/*metabolism/pharmacology
MH  - Thrombosis/genetics/metabolism
MH  - Vascular Cell Adhesion Molecule-1/genetics
OTO - NOTNLM
OT  - Caveolin-1
OT  - Endothelial cells
OT  - Inflammation
OT  - MALDI-TOF mass spectrometry
OT  - PEDF
EDAT- 2013/10/29 06:00
MHDA- 2014/01/18 06:00
CRDT- 2013/10/29 06:00
PHST- 2013/09/30 00:00 [received]
PHST- 2013/10/15 00:00 [accepted]
PHST- 2013/10/29 06:00 [entrez]
PHST- 2013/10/29 06:00 [pubmed]
PHST- 2014/01/18 06:00 [medline]
AID - S0006-291X(13)01755-5 [pii]
AID - 10.1016/j.bbrc.2013.10.074 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Nov 15;441(2):405-10. doi: 
      10.1016/j.bbrc.2013.10.074. Epub 2013 Oct 23.