PMID- 24161994
OWN - NLM
STAT- MEDLINE
DCOM- 20140822
LR  - 20161128
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 150
IP  - 3
DP  - 2013 Dec 12
TI  - Da-Huang-Fu-Zi-Tang attenuates liver injury in rats with severe acute 
      pancreatitis.
PG  - 960-6
LID - S0378-8741(13)00702-2 [pii]
LID - 10.1016/j.jep.2013.09.051 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Da-Huang-Fu-Zi-Tang (DHFZT) is a famous 
      traditional Chinese prescription with strong anti-inflammatory effects. Our 
      previous work found that DHFZT could act against pancreatic injury in rats with 
      severe acute pancreatitis (SAP) via inhibiting the Janus kinase 2/signal 
      transducers and activators of transcription 3 (JAK2/STAT3) signaling pathway in 
      pancreatic tissues. AIM OF THE STUDY: To investigate the therapeutic effects of 
      DHFZT on liver injury in SAP rats, and the effects on JAK2/STAT3 signaling in 
      liver tissue and Kupffer cells (KCs). MATERIALS AND METHODS: Fifty SD male rats 
      were randomly divided into five groups: sham operation group (SO), SAP model 
      group, DHFZT treatment groups (12, 24, and 48 mg/kg body weight). The model of 
      SAP was constructed by injecting sodium taurocholate (3.5%) into pancreatic and 
      biliary ducts. One hour before constructing the model, DHFZT was perfused into 
      the stomach. All rats were sacrificed after 24h following the operation; livers 
      were examined with hematoxylin and eosin staining. The protein expression of 
      pJAK2 and pSTAT3 in liver tissue was detected by immunohistochemical staining. 
      The activity of ALT, IL-6 and TNF-alpha in serum was detected. KCs of each group were 
      isolated. After culture for 4h, the protein expression of JAK2, pJAK2, STAT3 and 
      pSTAT3, the mRNA expression of IL-6 and TNF-alpha in KCs were examined. RESULTS: 
      Sodium taurocholate induced liver injury concomitant with increased expression of 
      pJAK2 and pSTAT3 in liver tissue and KCs. Pretreatment with DHFZT significantly 
      attenuated liver injury induced by SAP, and concurrently, effectively lowered the 
      serum ALT level. Furthermore, our studies showed that DHFZT obviously decreased 
      the expression of pJAK2 and pSTAT3 in liver tissue and KCs. CONCLUSIONS: DHFZT 
      could ameliorate liver injury in rats with SAP.
CI  - (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Wu, Li
AU  - Wu L
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, 
      Nanjing 210023, PR China; Engineering Center of State Ministry of Education for 
      Standardization of Chinese Medicine Processing, Nanjing University of Chinese 
      Medicine, Nanjing 210023, PR China; National First-Class Key Discipline for 
      Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, 
      Nanjing 210023, PR China.
FAU - Li, Huan
AU  - Li H
FAU - Zheng, Shi-zhong
AU  - Zheng SZ
FAU - Liu, Xiao
AU  - Liu X
FAU - Cai, Hao
AU  - Cai H
FAU - Cai, Bao-chang
AU  - Cai BC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131024
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Da-Huang-Fu-Zi-Tang)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-6)
RN  - 0 (Protective Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 5E090O0G3Z (Taurocholic Acid)
RN  - EC 2.7.10.2 (Jak2 protein, rat)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology/*therapeutic use
MH  - Cells, Cultured
MH  - Chemical and Drug Induced Liver Injury/*drug therapy/metabolism/pathology
MH  - Drugs, Chinese Herbal/pharmacology/*therapeutic use
MH  - Interleukin-6/blood/genetics
MH  - Janus Kinase 2/metabolism
MH  - Kupffer Cells/drug effects/metabolism
MH  - Liver/pathology
MH  - Male
MH  - Pancreatitis/*drug therapy/metabolism/pathology
MH  - Phytotherapy
MH  - Protective Agents/pharmacology/*therapeutic use
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - STAT3 Transcription Factor/metabolism
MH  - Taurocholic Acid
MH  - Tumor Necrosis Factor-alpha/blood/genetics
OTO - NOTNLM
OT  - ANOVA
OT  - Da-Huang-Fu-Zi-Tang
OT  - ELISA
OT  - HE
OT  - HPLC-MS
OT  - JAK2
OT  - JAK2/STAT3 pathway
OT  - Janus kinase 2
OT  - KCs
OT  - Kupffer cells
OT  - Liver injury
OT  - PBS
OT  - SAP
OT  - STAT3
OT  - Severe acute pancreatitis
OT  - TCM
OT  - analysis of variance
OT  - enzyme-linked immunosorbent assay
OT  - hematoxylin and eosin
OT  - high performance liquid chromatography-mass spectrometry
OT  - phosphate buffered saline
OT  - severe acute pancreatitis
OT  - signal transducers and activators of transcription 3
OT  - traditional Chinese medicine
EDAT- 2013/10/29 06:00
MHDA- 2014/08/26 06:00
CRDT- 2013/10/29 06:00
PHST- 2013/05/03 00:00 [received]
PHST- 2013/09/25 00:00 [revised]
PHST- 2013/09/26 00:00 [accepted]
PHST- 2013/10/29 06:00 [entrez]
PHST- 2013/10/29 06:00 [pubmed]
PHST- 2014/08/26 06:00 [medline]
AID - S0378-8741(13)00702-2 [pii]
AID - 10.1016/j.jep.2013.09.051 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2013 Dec 12;150(3):960-6. doi: 10.1016/j.jep.2013.09.051. Epub 
      2013 Oct 24.