PMID- 24162338
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131029
LR  - 20211021
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 93
IP  - 4
DP  - 1996 Sep
TI  - Molecular-cytogenetic characterization of a higher plant centromere/kinetochore 
      complex.
PG  - 477-84
LID - 10.1007/BF00417938 [doi]
AB  - The centromeric region of a telocentric field bean chromosome that resulted from 
      centric fission of the metacentric satellite chromosome was microdissected. The 
      DNA of this region was amplified and biotinylated by degenerate 
      oligonucleotide-primed polymerase chain reaction (DOP-PCR)/linker-adapter PCR. 
      After fluorescence in situ hybridization (FISH) the entire chromosome complement 
      of Vicia faba was labelled by these probes except for the nucleolus organizing 
      region (NOR) and the interstitial heterochromatin, the chromosomes of V. sativa 
      and V. narbonensis were only slightly labelled by the same probes. Dense uniform 
      labelling was also observed when a probe amplified from a clearly delimited 
      microdissected centromeric region of a mutant of Tradescantia paludosa was 
      hybridized to T. paludosa chromosomes. Even after six cycles of subtractive 
      hybridization between DNA fragments amplified from centromeric and acentric 
      regions no sequences specifically located at the field bean centromeres were 
      found among the remaining DNA. A mouse antiserum was produced which detected 
      nuclear proteins of 33 kDa and 68 kDa; these were predominantly located at V. 
      faba kinetochores during mitotic metaphase. DNA amplified from the chromatin 
      fraction adsorbed by this serum out of the sonicated total mitotic chromatin also 
      did not cause specific labelling of primary constrictions. From these results we 
      conclude: (1) either centromere-specific DNA sequences are not very conserved 
      among higher plants and are - at least in species with large genomes - 
      intermingled with complex dispersed repetitive sequences that prevent the 
      purification of the former, or (2) (some of) the dispersed repeats themselves 
      specify the primary constrictions by stereophysical parameters rather than by 
      their base sequence.
FAU - Houben, A
AU  - Houben A
AD  - Institut fur Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 
      D-06466, Gatersleben, Germany.
FAU - Brandes, A
AU  - Brandes A
FAU - Pich, U
AU  - Pich U
FAU - Manteuffel, R
AU  - Manteuffel R
FAU - Schubert, I
AU  - Schubert I
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 2013/10/29 06:00
PHST- 1996/01/12 00:00 [received]
PHST- 1996/01/19 00:00 [accepted]
PHST- 2013/10/29 06:00 [entrez]
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
AID - 10.1007/BF00417938 [doi]
PST - ppublish
SO  - Theor Appl Genet. 1996 Sep;93(4):477-84. doi: 10.1007/BF00417938.