PMID- 24162985
OWN - NLM
STAT- MEDLINE
DCOM- 20140521
LR  - 20151119
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1094
DP  - 2014
TI  - Ultrastructural and immunofluorescent methods for the study of the XY body as a 
      biomarker.
PG  - 137-49
LID - 10.1007/978-1-62703-706-8_11 [doi]
AB  - Structural and immunohistochemical methods have been extremely useful for the 
      characterization of the XY body (the structure formed by the XY pair during 
      meiotic prophase) in Man and in other mammals. These methods are widely used at 
      the present time for the detection of abnormalities leading to human infertility. 
      The basic ultrastructural methods are spreading of pachytene spermatocytes, 
      thin-sectioning techniques with or without 3-D reconstructions, and the 
      monitoring of all specimens with semi-thin sections. Immunofluorescent techniques 
      also use spreading of meiotic cells for the analysis of the XY body, and they can 
      be combined with the fluorescence in situ hybridization (FISH) technique, in the 
      so-called immuno-FISH. Epitope retrieval techniques are also used.
FAU - Sciurano, Roberta B
AU  - Sciurano RB
AD  - Departamento de Biologia Celular, Facultad de Medicina, Universidad de Buenos 
      Aires, Buenos Aires, Argentina.
FAU - Solari, Alberto J
AU  - Solari AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers)
RN  - 0 (Chromatin)
SB  - IM
MH  - Biomarkers/*metabolism
MH  - Biopsy
MH  - Chromatin/*ultrastructure
MH  - Fluorescent Antibody Technique/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Spermatocytes/ultrastructure
MH  - Staining and Labeling
MH  - Testis/pathology
EDAT- 2013/10/29 06:00
MHDA- 2014/05/23 06:00
CRDT- 2013/10/29 06:00
PHST- 2013/10/29 06:00 [entrez]
PHST- 2013/10/29 06:00 [pubmed]
PHST- 2014/05/23 06:00 [medline]
AID - 10.1007/978-1-62703-706-8_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1094:137-49. doi: 10.1007/978-1-62703-706-8_11.