PMID- 24166380
OWN - NLM
STAT- MEDLINE
DCOM- 20140522
LR  - 20181202
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1098
DP  - 2014
TI  - Simultaneous in vivo monitoring of regulatory and effector T lymphocytes using 
      secreted Gaussia luciferase, Firefly luciferase, and secreted alkaline 
      phosphatase.
PG  - 211-27
LID - 10.1007/978-1-62703-718-1_17 [doi]
AB  - Regulatory T cells (Tregs) are amongst the most widely studied cells in a variety 
      of immune-mediated conditions, including transplantation and Graft Versus Host 
      Disease (GVHD), cancer and autoimmunity; indeed, there is great interest in the 
      tolerogenic potential of Treg-based therapy. Consequently, the need to establish 
      the mechanisms that determine Treg survival and longevity, in addition to 
      developing new tools to monitor these parameters, is paramount. Using both a 
      mouse model of GVHD and a mouse model of Type 1 Diabetes (T1D), we describe 
      herein a dual reporter system based on Gluc and multiplexed with SEAP and 
      non-secreted Firefly luciferase (Fluc), which permits simultaneous imaging and 
      noninvasive tracking of two different T-cell populations (CD4(+)CD25(+) Tregs and 
      CD4(+)CD25(-) Tcon cells) in vivo by transducing the cells with different 
      lentiviruses bearing distinct color signatures. This new technology promises to 
      overcome the limitations of the conventional methods currently available to study 
      lymphocyte survival in vivo. Furthermore, this novel technique has applications 
      not only in autoimmunity and alloimmunity, but also in the wider field of 
      immunology.
FAU - Lewandrowski, Grant K
AU  - Lewandrowski GK
AD  - Experimental Therapeutics and Molecular Imaging Laboratory, Department of 
      Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.
FAU - Magee, Ciara N
AU  - Magee CN
FAU - Mounayar, Marwan
AU  - Mounayar M
FAU - Tannous, Bakhos A
AU  - Tannous BA
FAU - Azzi, Jamil
AU  - Azzi J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - EC 1.13.12.7 (Luciferases, Firefly)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/*metabolism
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Copepoda/*enzymology
MH  - Forkhead Transcription Factors/metabolism
MH  - Gene Expression Regulation/immunology
MH  - HEK293 Cells
MH  - Humans
MH  - Lentivirus/genetics
MH  - Luciferases, Firefly/genetics/*metabolism
MH  - *Luminescent Measurements
MH  - Mice
MH  - Molecular Imaging/*methods
MH  - Precursor Cells, T-Lymphoid/cytology
MH  - T-Lymphocytes, Helper-Inducer/*cytology/metabolism
MH  - T-Lymphocytes, Regulatory/*cytology/metabolism
MH  - Time Factors
EDAT- 2013/10/30 06:00
MHDA- 2014/05/23 06:00
CRDT- 2013/10/30 06:00
PHST- 2013/10/30 06:00 [entrez]
PHST- 2013/10/30 06:00 [pubmed]
PHST- 2014/05/23 06:00 [medline]
AID - 10.1007/978-1-62703-718-1_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1098:211-27. doi: 10.1007/978-1-62703-718-1_17.