PMID- 24166477
OWN - NLM
STAT- MEDLINE
DCOM- 20150226
LR  - 20220317
IS  - 1573-6814 (Electronic)
IS  - 1389-9333 (Linking)
VI  - 15
IP  - 2
DP  - 2014 Jun
TI  - In toto differentiation of human amniotic membrane towards the Schwann cell 
      lineage.
PG  - 227-39
LID - 10.1007/s10561-013-9401-1 [doi]
AB  - Human amniotic membrane (hAM) is a tissue containing cells with proven stem cell 
      properties. In its decellularized form it has been successfully applied as nerve 
      conduit biomaterial to improve peripheral nerve regeneration in injury models. We 
      hypothesize that viable hAM without prior cell isolation can be differentiated 
      towards the Schwann cell lineage to generate a possible alternative to commonly 
      applied tissue engineering materials for nerve regeneration. For in vitro Schwann 
      cell differentiation, biopsies of hAM of 8 mm diameter were incubated with a 
      sequential order of neuronal induction and growth factors for 21 days and 
      characterized for cellular viability and the typical glial markers glial 
      fibrillary acidic protein (GFAP), S100beta, p75 and neurotrophic tyrosine kinase 
      receptor (NTRK) using immunohistology. The secretion of the neurotrophic factors 
      brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic 
      factor (GDNF) was quantified by ELISA. The hAM maintained high viability, 
      especially under differentiation conditions (90.2 % +/- 41.6 day 14; 80.0 % +/- 44.5 
      day 21 compared to day 0). Both, BDNF and GDNF secretion was up-regulated upon 
      differentiation. The fresh membrane stained positive for GFAP and p75 and NTRK, 
      which was strongly increased after culture in differentiation conditions. 
      Especially the epithelial layer within the membrane exhibited a change in 
      morphology upon differentiation forming a multi-layered epithelium with intense 
      accumulations of the marker proteins. However, S100beta was expressed at equal 
      levels and equal distribution in fresh and cultured hAM conditions. Viable hAM 
      may be a promising alternative to present formulations used for peripheral nerve 
      regeneration.
FAU - Banerjee, Asmita
AU  - Banerjee A
AD  - Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA 
      Research Center, Donaueschingenstrasse 13, 1200, Vienna, Austria.
FAU - Nurnberger, Sylvia
AU  - Nurnberger S
FAU - Hennerbichler, Simone
AU  - Hennerbichler S
FAU - Riedl, Sabrina
AU  - Riedl S
FAU - Schuh, Christina M A P
AU  - Schuh CM
FAU - Hacobian, Ara
AU  - Hacobian A
FAU - Teuschl, Andreas
AU  - Teuschl A
FAU - Eibl, Johann
AU  - Eibl J
FAU - Redl, Heinz
AU  - Redl H
FAU - Wolbank, Susanne
AU  - Wolbank S
LA  - eng
PT  - Journal Article
DEP - 20131029
PL  - Netherlands
TA  - Cell Tissue Bank
JT  - Cell and tissue banking
JID - 100965121
SB  - IM
MH  - Amnion/*cytology/metabolism
MH  - Cell Differentiation/*physiology
MH  - Cell Lineage/*physiology
MH  - *Cell Separation
MH  - Cells, Cultured
MH  - Humans
MH  - Regeneration/physiology
MH  - Schwann Cells/*cytology
MH  - Stem Cells/cytology
EDAT- 2013/10/30 06:00
MHDA- 2015/02/27 06:00
CRDT- 2013/10/30 06:00
PHST- 2013/07/31 00:00 [received]
PHST- 2013/10/01 00:00 [accepted]
PHST- 2013/10/30 06:00 [entrez]
PHST- 2013/10/30 06:00 [pubmed]
PHST- 2015/02/27 06:00 [medline]
AID - 10.1007/s10561-013-9401-1 [doi]
PST - ppublish
SO  - Cell Tissue Bank. 2014 Jun;15(2):227-39. doi: 10.1007/s10561-013-9401-1. Epub 
      2013 Oct 29.