PMID- 24167062
OWN - NLM
STAT- MEDLINE
DCOM- 20140722
LR  - 20211021
IS  - 1097-0339 (Electronic)
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 42
IP  - 1
DP  - 2014 Jan
TI  - Cytologic features in vitreous preparations of patients with suspicion of 
      intraocular lymphoma.
PG  - 37-44
LID - 10.1002/dc.23059 [doi]
AB  - Intraocular lymphoma may occur, primarily with or without overt parenchymal CNS 
      lymphoma or secondarily from a variety of other lymphomas. The diagnosis is 
      frequently based on cytologic features and/or a limited panel of ancillary 
      techniques. Twenty-seven cytology samples from the vitreous preparations of 20 
      patients with clinical suspicion or histopathologic diagnoses of lymphoma were 
      retrospectively reviewed (2007-2011). Floaters and decreased visual acuity were 
      the most common ocular symptoms. Sixteen (of 20) patients had at least one 
      cytology that was "positive" or "suspicious" for lymphoma (F = 10; M = 6, median 
      age = 71 years, range = 52-82). Involvement was limited to the eye in half of the 
      cases (n = 8). Seven patients had CNS involvement, and three had systemic 
      lymphoma. Cytologic specimens were classified as large B cell lymphoma (LBCL) (n 
      = 13), suspicious for LBCL (n = 5), atypical/negative (n = 5), and consistent 
      with NK-T cell lymphoma (n = 1). Three cytologic samples were acellular. 
      Cytologic findings included increased cellularity (high n = 7, moderate n = 7), 
      large to medium (n = 19) cell size, marked nuclear irregularities (n = 12), 
      frequent apoptosis (n = 7), lymphoglandular bodies (n = 12), and necrosis (n = 
      12). Easily identifiable mitotic figures were present in two cases. CD20 
      immunocytochemistry performed in 15 cases with available cell blocks/destained 
      cytospins preparations, was confirmatory in 8 cases. Cytologic evaluation of 
      intraocular lymphoma is possible in vitreous specimens. Extraocular involvement 
      was not present in half of the cases. The most useful cytologic features include 
      increased cellularity, necrosis, and nuclear enlargement. Ancillary 
      immunocytochemical studies are useful and support the morphologic impression in a 
      subset of cases.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Rodriguez, Erika F
AU  - Rodriguez EF
AD  - Department of Pathology, Johns Hopkins University, Baltimore, Maryland.
FAU - Sepah, Yasir J
AU  - Sepah YJ
FAU - Jang, Hyun Soo
AU  - Jang HS
FAU - Ibrahim, Mohamed
AU  - Ibrahim M
FAU - Nguyen, Quan Dong
AU  - Nguyen QD
FAU - Rodriguez, Fausto J
AU  - Rodriguez FJ
LA  - eng
GR  - P30 CA006973/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20131025
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Needle
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Intraocular Lymphoma/*pathology
MH  - Lymphoma, B-Cell/pathology
MH  - Lymphoma, Extranodal NK-T-Cell/pathology
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Vitreous Body/*pathology
PMC - PMC3991129
MID - NIHMS565709
OTO - NOTNLM
OT  - CNS lymphoma
OT  - floaters
OT  - intraocular lymphoma
OT  - lymphoma
OT  - optical coherence tomography
OT  - vitreous cytology
OT  - vitritis
EDAT- 2013/10/30 06:00
MHDA- 2014/07/23 06:00
PMCR- 2014/04/18
CRDT- 2013/10/30 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/08/24 00:00 [revised]
PHST- 2013/10/03 00:00 [accepted]
PHST- 2013/10/30 06:00 [entrez]
PHST- 2013/10/30 06:00 [pubmed]
PHST- 2014/07/23 06:00 [medline]
PHST- 2014/04/18 00:00 [pmc-release]
AID - 10.1002/dc.23059 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2014 Jan;42(1):37-44. doi: 10.1002/dc.23059. Epub 2013 Oct 25.