PMID- 24169339
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20231213
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 93
IP  - 28
DP  - 2013 Jul 23
TI  - [Establishment of enzyme-linked immunosorbent assay in the detection of 
      BP180NC16A-specific IgE and its significance in bullous pemphigoid].
PG  - 2244-7
AB  - OBJECTIVE: To establish a method of detecting circulating immunoglobulin E (IgE) 
      autoantibodies for BP180NC16A and evaluate its significance in bullous pemphigoid 
      (BP). METHODS: GST-NC16A fusion proteins were expressed in Escherichia coli using 
      the pGEX-2T expression system and purified by glutathione affinity 
      chromatography.For optimal working conditions of enzyme-linked immunoabsorbent 
      assay (ELISA), checkerboard titrations were performed with serial dilutions of 
      antigen. Also optimized dilution of secondary antibody was confirmed. Sera 
      samples from 56 patients with BP, 24 healthy controls, 18 with pemphigus and 1 
      with Stevens-Johnson syndrome at our hospital during February 2011 to October 
      2012 were examined by the modified ELISA approach. The optimal cut-off point for 
      a positive result was selected with receiver operating characteristic analysis. 
      RESULTS: The optimized ELISA was performed with plates coated with 500 microg/ml 
      GST-NC16A. And the optimal dilutions of sera samples and secondary antibody were 
      1: 10 and 1: 1000 respectively. According to the established cut-off value 
      (0.549), 40 of 56 BP patients and none of controls had detectable levels of 
      BP180NC16A IgE. CONCLUSION: The established ELISA provides a highly specific tool 
      for the detection of IgE anti-BP180NC16A in BP patients.
FAU - Liu, Bing
AU  - Liu B
AD  - Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
FAU - Zuo, Ya-gang
AU  - Zuo YG
FAU - Zhou, Xi-ping
AU  - Zhou XP
FAU - He, Chun-xia
AU  - He CX
FAU - Li, Jun
AU  - Li J
FAU - Tie, Duerna
AU  - Tie D
FAU - Li, Li
AU  - Li L
FAU - Sun, Qiu-ning
AU  - Sun QN
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Autoantigens)
RN  - 0 (Non-Fibrillar Collagens)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Autoantigens/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Non-Fibrillar Collagens/*immunology
MH  - Recombinant Proteins/immunology
MH  - Collagen Type XVII
EDAT- 2013/10/31 06:00
MHDA- 2015/05/15 06:00
CRDT- 2013/10/31 06:00
PHST- 2013/10/31 06:00 [entrez]
PHST- 2013/10/31 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2013 Jul 23;93(28):2244-7.