PMID- 24171343
OWN - NLM
STAT- MEDLINE
DCOM- 20140424
LR  - 20181202
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 27
IP  - 8
DP  - 2013 Aug
TI  - [Protective effect of bone marrow mesenchymal stem cells on islets from 
      hypoxia/reoxygenation-induced injury].
PG  - 910-5
AB  - OBJECTIVE: To study the protective effects of bone marrow mesenchymal stem cells 
      (BMSCs) of rhesus monkeys on porcine islets from hypoxia/reoxygenation 
      (H/R)-induced injury. METHODS: BMSCs were isolated and cultured from the marrow 
      of 5 adult rhesus monkeys (weighing, 6-10 kg) by adherent monocytes. Islets were 
      isolated and purified from the pancreas of 5 neonatal porcine (3-5 days old) by 
      collagenase V digestion method, and were cultured with or without BMSCs, and 
      exposed to hypoxia (1% O2) for 12 hours and reoxygenation for 24 or 48 hours, 
      respectively. The experiment was divided into 4 groups: normal islet group (group 
      A), normal islet + BMSCs group (Group B), H/R islet group (group C), and H/R 
      islet + BMSCs group (group D). The survival rate of islets was calculated by 
      fluorescein diacetate/propidium iodide (PI) staining. The viability of the islet 
      cells was detected by cell counting kit 8. Apoptotic rate of islet cells was 
      tested using Annexin V-FITC/PI labeling and flow cytometry. The stimulation index 
      (SI) of islet function was analyzed by glucose-stimulated insulin secretion 
      assay. RESULTS: The islet cell cluster of group C was more dispersed than that of 
      groups A and B, and group C had more death cells; and the islet cell cluster of 
      group D was more complete and the survival rate was higher than those of group C. 
      The survival rate of islet was 90.2% +/- 9.1%, 88.3% +/- 5.9%, 52.3% +/- 12.1%, 
      and 71.4% +/- 11.5% in groups A, B, C, and D respectively, it was significantly 
      lower in groups C and D than in groups A and B (P < 0.05), but it was 
      significantly higher in group D than in group C (P < 0.05). After coculture of 
      BMSCs and islet at the ratio of 1:10 and 1:20 in group D, the viability of islet 
      cells was significantly higher than that in group C (P < 0.05). The apoptotic 
      rate was 27.1% +/- 3.2%, 24.0% +/- 1.0%, 64.3% +/- 1.8%, and 46.2% +/- 1.4% in 
      groups A, B, C, and D respectively, it was significantly higher in groups C and D 
      than that in groups A and B (P < 0.05), but it was significantly lower in group D 
      than in group C (P < 0.05). There was no significant difference in SI between 
      groups A and B at each time point (P > 0.05), but it was significantly lower in 
      group C than in groups A and B (P < 0.05); and it was significantly higher in 
      group D than in group C at 24 and 72 hours (P < 0.05). CONCLUSION: BMSCs of 
      rhesus monkeys can protect islet vitality and function from H/R-induced injury.
FAU - Zhao, Jiuming
AU  - Zhao J
AD  - Ministry of Health, Regenerative Medicine Research Center, West China Hospital, 
      Sichuan University, Chengdu Sichuan, 610041, P R China.
FAU - He, Sirong
AU  - He S
FAU - Wang, Chengshi
AU  - Wang C
FAU - Liu, Jingping
AU  - Liu J
FAU - Li, Shengfu
AU  - Li S
FAU - Chen, Younan
AU  - Chen Y
FAU - Lu, Yanrong
AU  - Lu Y
FAU - Cheng, Jingqiu
AU  - Cheng J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Insulin)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Cell Hypoxia
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Female
MH  - Insulin/metabolism
MH  - Islets of Langerhans/cytology/pathology/*physiology
MH  - Islets of Langerhans Transplantation
MH  - Macaca mulatta
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*physiology
MH  - Oxygen/*administration & dosage
MH  - Swine
MH  - Tissue Engineering/methods
EDAT- 2013/11/01 06:00
MHDA- 2014/04/25 06:00
CRDT- 2013/11/01 06:00
PHST- 2013/11/01 06:00 [entrez]
PHST- 2013/11/01 06:00 [pubmed]
PHST- 2014/04/25 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2013 Aug;27(8):910-5.