PMID- 24172282
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140429
LR  - 20211021
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 13
IP  - 11
DP  - 2013 Oct 29
TI  - Combining electrochemical impedance spectroscopy and surface plasmon resonance 
      into one simultaneous read-out system for the detection of surface interactions.
PG  - 14650-61
LID - 10.3390/s131114650 [doi]
AB  - In this article we describe the integration of impedance spectroscopy (EIS) and 
      surface plasmon resonance (SPR) into one surface analytic device. A 
      polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a 
      commercially available sensor chip used for SPR measurements. This flow cell 
      allowed simultaneous measurements between an EIS and a SPR setup. After a 
      successful integration, a proof of principle study was conducted to investigate 
      any signs of interference between the two systems during a measurement. The flow 
      cell was rinsed with 10 mM Tris-HCl and 1x PBS buffer in an alternating manner, 
      while impedance and shifts of the resonance angle were monitored. After achieving 
      a successful proof of principle, a usability test was conducted. It was assessed 
      whether simultaneous detection occurred when: (i) Protein A is adsorbed to the 
      gold surface of the chip; (ii) The non-occupied zone is blocked with BSA 
      molecules and (iii) IgG1 is bound to the Protein A. The results indicate a 
      successful merge between SPR and EIS.
FAU - Vandenryt, Thijs
AU  - Vandenryt T
AD  - Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, 3590 
      Diepenbeek, Belgium. thijs.vandenryt@uhasselt.be.
FAU - Pohl, Andrea
AU  - Pohl A
FAU - van Grinsven, Bart
AU  - van Grinsven B
FAU - Thoelen, Ronald
AU  - Thoelen R
FAU - De Ceuninck, Ward
AU  - De Ceuninck W
FAU - Wagner, Patrick
AU  - Wagner P
FAU - Opitz, Jorg
AU  - Opitz J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131029
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
PMC - PMC3871058
EDAT- 2013/11/01 06:00
MHDA- 2013/11/01 06:01
PMCR- 2013/11/01
CRDT- 2013/11/01 06:00
PHST- 2013/08/28 00:00 [received]
PHST- 2013/09/24 00:00 [revised]
PHST- 2013/10/03 00:00 [accepted]
PHST- 2013/11/01 06:00 [entrez]
PHST- 2013/11/01 06:00 [pubmed]
PHST- 2013/11/01 06:01 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - s131114650 [pii]
AID - sensors-13-14650 [pii]
AID - 10.3390/s131114650 [doi]
PST - epublish
SO  - Sensors (Basel). 2013 Oct 29;13(11):14650-61. doi: 10.3390/s131114650.