PMID- 24173043 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20131101 LR - 20211021 IS - 0040-5752 (Print) IS - 0040-5752 (Linking) VI - 90 IP - 7-8 DP - 1995 Jun TI - A chromosome 5-specific repetitive DNA sequence in rice (Oryza sativa L). PG - 907-13 LID - 10.1007/BF00222902 [doi] AB - Repetitive DNA sequences in the rice genome comprise more than half of the nuclear DNA. The isolation and characterization of these repetitive DNA sequences should lead to a better understanding of rice chromosome structure and genome organization. We report here the characterization and chromosome localization of a chromosome 5-specific repetitive DNA sequence. This repetitive DNA sequence was estimated to have at least 900 copies. DNA sequence analysis of three genomic clones which contain the repeat unit indicated that the DNA sequences have two sub-repeat units of 37 bp and 19 bp, connected by 30-to 90-bp short sequences with high similarity. RFLP mapping and physical mapping by fluorescence in situ hybridization (FISH) indicated that almost all copies of the repetitive DNA sequence are located in the centromeric heterochromatic region of the long arm of chromosome 5. The strategy for cloning such repetitive DNA sequences and their uses in rice genome research are discussed. FAU - Wang, Z X AU - Wang ZX AD - Rice Genome Research Program, National Institute of Agrobiological Resources /STAFF (Society for Techno-innovation of Agriculture, Forestry and Fisheries) Institute, 2-1-2 Kannondai, Tsukuba, 305, Ibaraki, Japan. FAU - Kurata, N AU - Kurata N FAU - Saji, S AU - Saji S FAU - Katayose, Y AU - Katayose Y FAU - Minobe, Y AU - Minobe Y LA - eng PT - Journal Article PL - Germany TA - Theor Appl Genet JT - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JID - 0145600 EDAT- 1995/06/01 00:00 MHDA- 1995/06/01 00:01 CRDT- 2013/11/01 06:00 PHST- 1994/08/04 00:00 [received] PHST- 1994/09/30 00:00 [accepted] PHST- 2013/11/01 06:00 [entrez] PHST- 1995/06/01 00:00 [pubmed] PHST- 1995/06/01 00:01 [medline] AID - 10.1007/BF00222902 [doi] PST - ppublish SO - Theor Appl Genet. 1995 Jun;90(7-8):907-13. doi: 10.1007/BF00222902.