PMID- 24174309
OWN - NLM
STAT- MEDLINE
DCOM- 20140724
LR  - 20211025
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 38
IP  - 1
DP  - 2014 Jan
TI  - Proteinase-activated receptor 2 modulates corticotropin releasing hormone-induced 
      brain-derived neurotrophic factor release from microglial cells.
PG  - 92-6
LID - 10.1002/cbin.10185 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) plays a critical role in the 
      pathogenesis of neuropathic pain, but its regulation of BDNF release is not fully 
      understood. To further understand the regulation of BDNF release, the microglial 
      cell line, C8-D1A (microglia, in short), were cultured as a model. The levels of 
      BDNF were determined by enzyme-linked immunoassay. Apoptotic microglia were 
      assessed by flow cytometry. The protease-activated receptor 2 (PAR2) was 
      activated by tryptase. Exposure to corticotripin releasing hormone (CRH) induced 
      BDNF release from microglia. Apoptosis was evident in microglia after activation 
      by CRH. Tryptase-induced PAR2 activation reduced the frequency of apoptosis of 
      microglia, but enhanced the BDNF levels in the culture medium, which was 
      partially blocked by PAR2 antagonists. We conclude that PAR2 agonists can promote 
      the BDNF release from microglia; the PAR2 antagonists may be a potential 
      therapeutic target to attenuate the BDNF-related neuropathic pain.
CI  - (c) 2013 International Federation for Cell Biology.
FAU - Fan, Yongzhi
AU  - Fan Y
AD  - Department of Anesthesiology and Department of Pain Medicine, The Central 
      Hospital of Wuhan, 26 Shengli St., Jiangan District, Wuhan, 430014, China.
FAU - Chen, Jingli
AU  - Chen J
FAU - Ye, Jun
AU  - Ye J
FAU - Yan, Hong
AU  - Yan H
FAU - Cai, Yi
AU  - Cai Y
LA  - eng
PT  - Journal Article
DEP - 20131030
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptor, PAR-2)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Line
MH  - Corticotropin-Releasing Hormone/*pharmacology
MH  - Mice
MH  - Microglia/cytology/metabolism
MH  - Receptor, PAR-2/agonists/antagonists & inhibitors/*metabolism
MH  - Toll-Like Receptor 4/metabolism
MH  - Tryptases/metabolism
OTO - NOTNLM
OT  - apoptosis
OT  - brain-derived neurotropin factor
OT  - corticotropin releasing hormone
OT  - microglia
OT  - protease-activated receptor 2
EDAT- 2013/11/01 06:00
MHDA- 2014/07/25 06:00
CRDT- 2013/11/01 06:00
PHST- 2013/06/22 00:00 [received]
PHST- 2013/08/11 00:00 [accepted]
PHST- 2013/11/01 06:00 [entrez]
PHST- 2013/11/01 06:00 [pubmed]
PHST- 2014/07/25 06:00 [medline]
AID - 10.1002/cbin.10185 [doi]
PST - ppublish
SO  - Cell Biol Int. 2014 Jan;38(1):92-6. doi: 10.1002/cbin.10185. Epub 2013 Oct 30.