PMID- 24175018
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 3
IP  - 7
DP  - 2010 Jul 7
TI  - Imaging Analysis of Carbohydrate-Modified Surfaces Using ToF-SIMS and SPRi.
PG  - 3948-64
LID - 10.3390/ma3073948 [doi]
AB  - Covalent modification of surfaces with carbohydrates (glycans) is a prerequisite 
      for a variety of glycomics-based biomedical applications, including functional 
      biomaterials, glycoarrays, and glycan-based biosensors. The chemistry of glycan 
      immobilization plays an essential role in the bioavailability and function of the 
      surface bound carbohydrate moiety. However, the scarcity of analytical methods to 
      characterize carbohydrate-modified surfaces complicates efforts to optimize 
      glycan surface chemistries for specific applications. Time-of-Flight Secondary 
      Ion Mass Spectrometry (ToF-SIMS) is a surface sensitive technique suited for 
      probing molecular composition at the biomaterial interface. Expanding ToF-SIMS 
      analysis to interrogate carbohydrate-modified materials would increase our 
      understanding of glycan surface chemistries and advance novel tools in the 
      nascent field of glycomics. In this study, a printed glycan microarray surface 
      was fabricated and subsequently characterized by ToF-SIMS imaging analysis. A 
      multivariate technique based on principal component analysis (PCA) was used to 
      analyze the ToF-SIMS dataset and reconstruct ToF-SIMS images of functionalized 
      surfaces. These images reveal chemical species related to the immobilized glycan, 
      underlying glycan-reactive chemistries, gold substrates, and outside 
      contaminants. Printed glycoarray elements (spots) were also interrogated to 
      resolve the spatial distribution and spot homogeneity of immobilized glycan. The 
      bioavailability of the surface-bound glycan was validated using a specific 
      carbohydrate-binding protein (lectin) as characterized by Surface Plasmon 
      Resonance Imaging (SPRi). Our results demonstrate that ToF-SIMS is capable of 
      characterizing chemical features of carbohydrate-modified surfaces and, when 
      complemented with SPRi, can play an enabling role in optimizing glycan microarray 
      fabrication and performance.
FAU - Bolles, Kathryn M
AU  - Bolles KM
AD  - Whitman College, 345 Boyer Ave., Walla Walla, WA 99362: USA.
FAU - Cheng, Fang
AU  - Cheng F
FAU - Burk-Rafel, Jesse
AU  - Burk-Rafel J
FAU - Dubey, Manish
AU  - Dubey M
FAU - Ratner, Daniel M
AU  - Ratner DM
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC3809004
MID - NIHMS523822
OTO - NOTNLM
OT  - SPR
OT  - ToF-SIMS
OT  - carbohydrate microarray
OT  - glycomics
EDAT- 2010/07/07 00:00
MHDA- 2010/07/07 00:01
PMCR- 2010/07/07
CRDT- 2013/11/01 06:00
PHST- 2013/11/01 06:00 [entrez]
PHST- 2010/07/07 00:00 [pubmed]
PHST- 2010/07/07 00:01 [medline]
PHST- 2010/07/07 00:00 [pmc-release]
AID - materials-03-03948 [pii]
AID - 10.3390/ma3073948 [doi]
PST - ppublish
SO  - Materials (Basel). 2010 Jul 7;3(7):3948-64. doi: 10.3390/ma3073948.