PMID- 24179694
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131101
LR  - 20211021
IS  - 2093-3665 (Print)
IS  - 2093-3673 (Electronic)
IS  - 2093-3665 (Linking)
VI  - 46
IP  - 3
DP  - 2013 Sep
TI  - Stereological study of the effects of morphine consumption and abstinence on the 
      number of the neurons and oligodendrocytes in medial prefrontal cortex of rats.
PG  - 191-7
LID - 10.5115/acb.2013.46.3.191 [doi]
AB  - Quantitative studies to date on the effects of opioid consumption and abstinence 
      on the nervous system using modern stereological methods have not received enough 
      attention. In addition, they have yielded controversial results. The present 
      study was conducted to investigate the effects of morphine, with or without 
      abstinence, on the neurons and oligodendrocytes of the medial prefrontal cortex 
      (MPFC) in rats using quantitative stereological methods. The male rats were 
      divided into four groups: the first (saline [SAL]) and second (morphine [MOR]) 
      groups were treated with saline and an escalating dose of morphine (5-20 mg/kg) 
      for 30 days, respectively; the third (SAL+abstinence [ABS]) and fourth (MOR+ABS) 
      groups were treated in the same manner as the previous groups plus they had a 
      30-day abstinence period. The results showed that the volume of the MPFC and its 
      subdivisions decreased by approximately 15% in the MOR group compared with that 
      in the SAL group (P<0.05). In addition, the volume decreased by approximately 24% 
      in the MOR+ABS group compared with that in the SAL+ABS group (P<0.05). The number 
      of neurons in the MOR and MOR+ABS groups decreased by approximately 44% and 35%, 
      respectively, compared with that in their corresponding control groups. Moreover, 
      the number of the oligodendrocytes in the MOR and MOR+ABS groups decreased by 
      approximately 41% and 37%, respectively. No significant difference was noted in 
      the number of cells in the MOR and MOR+ABS groups. In conclusion, morphine 
      consumption leads to a permanent reduction in the number of neurons and 
      oligodendrocytes, and no additional neuron and oligodendrocyte loss occurs after 
      abstinence.
FAU - Rafati, Ali
AU  - Rafati A
AD  - Histomorphometry and Stereology Research Center, Shiraz University of Medical 
      Sciences, Shiraz, Iran. ; Department of Physiology, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Noorafshan, Ali
AU  - Noorafshan A
FAU - Torabi, Nihad
AU  - Torabi N
LA  - eng
PT  - Journal Article
DEP - 20130930
PL  - Korea (South)
TA  - Anat Cell Biol
JT  - Anatomy & cell biology
JID - 101531987
PMC - PMC3811856
OTO - NOTNLM
OT  - Cortex
OT  - Morphine
OT  - Neurons
OT  - Oligodendroglia
OT  - Rat
OT  - Stereology
EDAT- 2013/11/02 06:00
MHDA- 2013/11/02 06:01
PMCR- 2013/09/01
CRDT- 2013/11/02 06:00
PHST- 2013/02/19 00:00 [received]
PHST- 2013/05/26 00:00 [revised]
PHST- 2013/06/04 00:00 [accepted]
PHST- 2013/11/02 06:00 [entrez]
PHST- 2013/11/02 06:00 [pubmed]
PHST- 2013/11/02 06:01 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - 10.5115/acb.2013.46.3.191 [doi]
PST - ppublish
SO  - Anat Cell Biol. 2013 Sep;46(3):191-7. doi: 10.5115/acb.2013.46.3.191. Epub 2013 
      Sep 30.