PMID- 24183726
OWN - NLM
STAT- MEDLINE
DCOM- 20140326
LR  - 20131125
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 441
IP  - 3
DP  - 2013 Nov 22
TI  - Cytokeratin 8-MHC class I interactions: a potential novel immune escape phenotype 
      by a lymph node metastatic carcinoma cell line.
PG  - 618-23
LID - S0006-291X(13)01786-5 [pii]
LID - 10.1016/j.bbrc.2013.10.105 [doi]
AB  - Defective human leukocyte antigen (HLA) class I expression in malignant cells 
      facilitates their escape from destruction by CD8(+) cytotoxic T lymphocytes. In 
      this study, a post-translational mechanism of HLA class I abnormality that does 
      not involve defects in the HLA subunits and antigen processing machinery 
      components was identified and characterized. The marked HLA class I 
      downregulation phenotype of a metastatic carcinoma cell line can be readily 
      reversed by trypsin, suggesting a masking effect by serine protease-sensitive HLA 
      class I-interacting factors. Co-immunoprecipitation, combined with LC-tandem mass 
      spectrometry and immunoblotting identified these factors as cytokeratin (CK) 8 
      and its heterodimeric partners CK18 and CK19. Ectopic CK8/18 or CK8/19 expression 
      in HEK293 cells resulted in surface CK8 expression with an HLA class I 
      downregulation phenotype, while redirecting CK8/18 and CK8/19 to the endoplasmic 
      reticulum (ER) had no such effect. This observation and the failure to constrain 
      CK8/18 and CK8/19 membrane trafficking by an ER-Golgi transport inhibitor 
      suggested an ER-independent route for CK8 access to HLA class I molecules. 
      Monoclonal antibody mapping revealed a potential CK8 blockade of HLA class I-CD8 
      and -TCR contacts. These findings, along with the emerging role of cell surface 
      CK8 in cancer metastasis, may imply a dual strategy for tumor cell survival in 
      the host.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Wu, Ming-Syue
AU  - Wu MS
AD  - Institute of Molecular and Cellular Biology, National Tsing Hua University, 
      Hsinchu, Taiwan.
FAU - Li, Chia-Hsuan
AU  - Li CH
FAU - Ruppert, Jan Gustav
AU  - Ruppert JG
FAU - Chang, Chien-Chung
AU  - Chang CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131030
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Keratin-18)
RN  - 0 (Keratin-19)
RN  - 0 (Keratin-8)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal
MH  - Carcinoma/*secondary
MH  - Cell Line, Tumor
MH  - HEK293 Cells
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Immunoprecipitation
MH  - Keratin-18
MH  - Keratin-19
MH  - Keratin-8/*immunology
MH  - Lymph Nodes/immunology/pathology
MH  - Lymphatic Metastasis/*immunology
MH  - Molecular Sequence Data
MH  - Protein Multimerization
MH  - Trypsin/immunology
MH  - *Tumor Escape
OTO - NOTNLM
OT  - Cytokeratin
OT  - Human leukocyte antigen
OT  - Tumor immune escape
EDAT- 2013/11/05 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/11/05 06:00
PHST- 2013/10/02 00:00 [received]
PHST- 2013/10/20 00:00 [accepted]
PHST- 2013/11/05 06:00 [entrez]
PHST- 2013/11/05 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - S0006-291X(13)01786-5 [pii]
AID - 10.1016/j.bbrc.2013.10.105 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Nov 22;441(3):618-23. doi: 
      10.1016/j.bbrc.2013.10.105. Epub 2013 Oct 30.