PMID- 24184357
OWN - NLM
STAT- MEDLINE
DCOM- 20140926
LR  - 20140113
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 447
DP  - 2014 Feb 15
TI  - Quantitation of heparosan with heparin lyase III and spectrophotometry.
PG  - 46-8
LID - S0003-2697(13)00509-5 [pii]
LID - 10.1016/j.ab.2013.10.029 [doi]
AB  - Heparosan is Escherichia coli K5 capsule polysaccharide, which is the key 
      precursor for preparing bioengineered heparin. A rapid and effective quantitative 
      method for detecting heparosan is important in the large-scale production of 
      heparosan. Heparin lyase III (Hep III) effectively catalyzes the heparosan 
      depolymerization, forming unsaturated disaccharides that are measurable using a 
      spectrophotometer at 232 nm. We report a new method for the quantitative 
      detection of heparosan with heparin lyase III and spectrophotometry that is safer 
      and more specific than the traditional carbazole assay. In an optimized detection 
      system, heparosan at a minimum concentration of 0.60 g/L in fermentation broth 
      can be detected.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Huang, Haichan
AU  - Huang H
AD  - College of Biological and Environmental Engineering, Zhejiang University of 
      Technology, Hangzhou 310032, People's Republic of China.
FAU - Zhao, Yingying
AU  - Zhao Y
AD  - College of Biological and Environmental Engineering, Zhejiang University of 
      Technology, Hangzhou 310032, People's Republic of China.
FAU - Lv, Shencong
AU  - Lv S
AD  - College of Biological and Environmental Engineering, Zhejiang University of 
      Technology, Hangzhou 310032, People's Republic of China.
FAU - Zhong, Weihong
AU  - Zhong W
AD  - College of Biological and Environmental Engineering, Zhejiang University of 
      Technology, Hangzhou 310032, People's Republic of China. Electronic address: 
      whzhong@zjut.edu.cn.
FAU - Zhang, Fuming
AU  - Zhang F
AD  - Department of Chemical and Biological Engineering, Rensselaer Polytechnic 
      Institute, Troy, NY 12180, USA.
FAU - Linhardt, Robert J
AU  - Linhardt RJ
AD  - Department of Chemical and Biological Engineering, Rensselaer Polytechnic 
      Institute, Troy, NY 12180, USA; Department of Biology, Rensselaer Polytechnic 
      Institute, Troy, NY 12180, USA; Departments of Chemistry and Chemical Biology and 
      Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, 
      Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131030
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Disaccharides)
RN  - 0 (heparosan)
RN  - EC 4.2.2.7 (Heparin Lyase)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Calibration
MH  - Disaccharides/*analysis/metabolism
MH  - Fermentation
MH  - Heparin Lyase/*metabolism
MH  - *Spectrophotometry
OTO - NOTNLM
OT  - Enzymatic method
OT  - Escherichia coli K5
OT  - Heparin lyase III
OT  - Heparosan
OT  - Quantitative method
EDAT- 2013/11/05 06:00
MHDA- 2014/09/27 06:00
CRDT- 2013/11/05 06:00
PHST- 2013/08/13 00:00 [received]
PHST- 2013/10/14 00:00 [revised]
PHST- 2013/10/21 00:00 [accepted]
PHST- 2013/11/05 06:00 [entrez]
PHST- 2013/11/05 06:00 [pubmed]
PHST- 2014/09/27 06:00 [medline]
AID - S0003-2697(13)00509-5 [pii]
AID - 10.1016/j.ab.2013.10.029 [doi]
PST - ppublish
SO  - Anal Biochem. 2014 Feb 15;447:46-8. doi: 10.1016/j.ab.2013.10.029. Epub 2013 Oct 
      30.