PMID- 24184564
OWN - NLM
STAT- MEDLINE
DCOM- 20140928
LR  - 20211203
IS  - 1873-1708 (Electronic)
IS  - 0890-6238 (Linking)
VI  - 43
DP  - 2014 Jan
TI  - Sub-chronic sulforaphane exposure in CD-1 pregnant mice enhances maternal NADPH 
      quinone oxidoreductase 1 (NQO1) activity and mRNA expression of NQO1, glutathione 
      S-transferase, and glutamate-cysteine ligase: potential implications for fetal 
      protection against toxicant exposure.
PG  - 30-7
LID - S0890-6238(13)00364-X [pii]
LID - 10.1016/j.reprotox.2013.10.009 [doi]
AB  - The study objective was to determine if maternal administration of sulforaphane 
      (SFN) induced Nrf2-controlled genes. In acute studies, when non-pregnant and 
      pregnant mice were orally exposed to SFN (50 or 100 mg/kg) on gestational day 
      (GD) 14 and euthanized after 2, 6 or 24h, results demonstrated increased GSTM1, 
      NQO1, HO-1, and Gclc mRNA transcript levels in adult liver, but no change in NQO1 
      activity. In sub-chronic studies, when non-pregnant and pregnant mice were orally 
      exposed to SFN (65 mg/kg) daily for 30 days and euthanized on GD14, results 
      demonstrated a 2- to 3-fold increase in GSTM1, Gclc and NQO1 transcript levels, 
      and a 2-fold increase in NQO1 activity in adult livers. No effects of maternal 
      treatment on fetal liver gene transcript levels or enzyme activity were observed. 
      Demonstration that SFN induces maternal gene expression and activity supports 
      further investigation of SFN as a preventative agent against transplacental 
      toxicity.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Philbrook, Nicola A
AU  - Philbrook NA
AD  - Department of Biomedical and Molecular Sciences, Graduate Program in Pharmacology 
      and Toxicology Graduate Program, Queen's University, Kingston, Ontario, Canada 
      K7L 3N6.
FAU - Winn, Louise M
AU  - Winn LM
AD  - Department of Biomedical and Molecular Sciences, Graduate Program in Pharmacology 
      and Toxicology Graduate Program, Queen's University, Kingston, Ontario, Canada 
      K7L 3N6; School of Environmental Studies, Queen's University, Kingston, Ontario, 
      Canada K7L 3N6. Electronic address: winnl@queensu.ca.
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131031
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (Isothiocyanates)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sulfoxides)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (Nqo1 protein, mouse)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.5.1.18 (glutathione S-transferase M1)
RN  - EC 6.3.2.2 (Glutamate-Cysteine Ligase)
RN  - GA49J4310U (sulforaphane)
SB  - IM
MH  - Animals
MH  - Anticarcinogenic Agents/*pharmacology
MH  - Female
MH  - Glutamate-Cysteine Ligase/genetics
MH  - Glutathione Transferase/genetics
MH  - Heme Oxygenase-1/genetics
MH  - Isothiocyanates/*pharmacology
MH  - Liver/drug effects/metabolism
MH  - Maternal-Fetal Exchange
MH  - Membrane Proteins/genetics
MH  - Mice
MH  - NAD(P)H Dehydrogenase (Quinone)/genetics/*metabolism
MH  - Pregnancy
MH  - RNA, Messenger/metabolism
MH  - Sulfoxides
OTO - NOTNLM
OT  - GST
OT  - Gclc
OT  - HO-1
OT  - NQO1
OT  - Sulforaphane
EDAT- 2013/11/05 06:00
MHDA- 2014/10/01 06:00
CRDT- 2013/11/05 06:00
PHST- 2013/07/22 00:00 [received]
PHST- 2013/10/16 00:00 [revised]
PHST- 2013/10/16 00:00 [accepted]
PHST- 2013/11/05 06:00 [entrez]
PHST- 2013/11/05 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
AID - S0890-6238(13)00364-X [pii]
AID - 10.1016/j.reprotox.2013.10.009 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2014 Jan;43:30-7. doi: 10.1016/j.reprotox.2013.10.009. Epub 2013 
      Oct 31.