PMID- 24185679
OWN - NLM
STAT- MEDLINE
DCOM- 20140410
LR  - 20211021
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 59
IP  - 2
DP  - 2014 Feb
TI  - Moderate physical exercise protects myenteric metabolically more active neurons 
      in mice infected with Trypanosoma cruzi.
PG  - 307-14
LID - 10.1007/s10620-013-2901-9 [doi]
AB  - BACKGROUND: Trypanosoma cruzi causes neuronal myenteric depopulation compromising 
      intestinal function. AIM: The purpose of this study was to evaluate the influence 
      of moderate physical exercise on NADH diaphorase (NADH-d)-positive neurons in the 
      myenteric plexus and intestinal wall of the colon in mice infected with T. cruzi. 
      MATERIALS AND METHODS: Forty 30-day-old male Swiss mice were divided into the 
      following groups: trained infected (TI), sedentary infected (SI), trained control 
      (TC), and sedentary control. The TC and TI groups were subjected to a moderate 
      physical exercise program on a treadmill for 8 weeks. Three days after finishing 
      physical exercise, the TI and SI groups were intraperitoneally inoculated with 
      1,300 blood trypomastigotes of the Y strain of Trypanosoma cruzi. Parasitemia was 
      evaluated from days 4 to 61 after inoculation. On day 75 of infection, myenteric 
      neurons in the colon were quantified (NADH-d), and inflammatory foci were 
      counted. Tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) 
      levels were evaluated in plasma. The results were compared using analysis of 
      variance and the Kruskal-Wallis test at a 5 % significance level. RESULTS: 
      Moderate physical exercise reduced the parasite peak on day 8 of infection 
      (p = 0.0132) and total parasitemia (p = 0.0307). It also prevented neuronal 
      depopulation (p < 0.01), caused hypertrophy of these cells (p < 0.05), prevented 
      the formation of inflammatory foci (p < 0.01), and increased the synthesis of 
      TNF-alpha (p < 0.01) and TGF-beta (p > 0.05). CONCLUSION: These results reinforce the 
      therapeutic benefits of moderate physical exercise for T. cruzi infection.
FAU - Moreira, Neide Martins
AU  - Moreira NM
AD  - Graduate Program in Health Sciences, State University of Maringa, Columbus 
      Avenue, 5790, Zone 07, Maringa, PR, 87020-900, Brazil, 
      neidemartinsenf@yahoo.com.br.
FAU - de Moraes, Solange Marta Franzoi
AU  - de Moraes SM
FAU - Dalalio, M M O
AU  - Dalalio MM
FAU - Gomes, Monica Lucia
AU  - Gomes ML
FAU - Sant'ana, D M G
AU  - Sant'ana DM
FAU - de Araujo, Silvana Marques
AU  - de Araujo SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131102
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Inflammation Mediators)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.8.1.4 (Dihydrolipoamide Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Chagas Disease/blood/parasitology/pathology/*therapy
MH  - Colon/*innervation
MH  - Dihydrolipoamide Dehydrogenase/metabolism
MH  - Disease Models, Animal
MH  - Hypertrophy
MH  - Inflammation Mediators/blood
MH  - Male
MH  - Mice
MH  - Myenteric Plexus/metabolism/*parasitology/pathology
MH  - Neurons/metabolism/*parasitology/pathology
MH  - *Physical Exertion
MH  - Time Factors
MH  - Transforming Growth Factor beta/blood
MH  - Trypanosoma cruzi/*pathogenicity
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2013/11/05 06:00
MHDA- 2014/04/11 06:00
CRDT- 2013/11/05 06:00
PHST- 2013/08/17 00:00 [received]
PHST- 2013/09/25 00:00 [accepted]
PHST- 2013/11/05 06:00 [entrez]
PHST- 2013/11/05 06:00 [pubmed]
PHST- 2014/04/11 06:00 [medline]
AID - 10.1007/s10620-013-2901-9 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2014 Feb;59(2):307-14. doi: 10.1007/s10620-013-2901-9. Epub 2013 Nov 
      2.