PMID- 24189292
OWN - NLM
STAT- MEDLINE
DCOM- 20140819
LR  - 20131230
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 77
DP  - 2014 Jan
TI  - Vietnamese Heterometrus laoticus scorpion venom: evidence for analgesic and 
      anti-inflammatory activity and isolation of new polypeptide toxin acting on Kv1.3 
      potassium channel.
PG  - 40-8
LID - S0041-0101(13)00413-3 [pii]
LID - 10.1016/j.toxicon.2013.10.027 [doi]
AB  - The scorpion Heterometrus laoticus (Scorpionidae) inhabits Indochinese peninsula 
      and is widely distributed in South-West Vietnam. Since no human fatalities caused 
      by H. laoticus stings were reported, no systematic characterization of the venom 
      was earlier done. In this study we report on biological activity of the venom 
      from H. laoticus caught in Vietnamese province An Giang. The venom manifested a 
      very low acute toxicity with LD50 of about 190 mg/kg body weight in mice at 
      subcutaneous (s.c.) injection and 12 mg/kg at intravenous injection. The venom 
      analgesic effects using tail immersion and writhing tests as well as 
      anti-inflammatory effect using carrageenan test were analyzed at doses of 9.5 and 
      19 mg/kg at s.c. injections. It was found that at two doses tested H. laoticus 
      venom showed both anti-nociceptive and anti-inflammatory activity. The venom was 
      fractionated by means of gel-filtration and reversed-phase HPLC. As a result 
      several polypeptide toxins were isolated and new toxin hetlaxin was identified. 
      Its amino acid sequence was determined and binding to the extracellular vestibule 
      of the K(+)-conducting pore of Kv1.1 and Kv1.3 potassium channels was studied. 
      Hetlaxin belongs to the scorpion alpha-toxin family and is the first toxin 
      isolated from H. laoticus venom which possesses high affinity (K(i) 59 nM) to 
      Kv1.3 potassium channel.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Hoang, Anh N
AU  - Hoang AN
AD  - Institute of Applied Materials Science, Vietnam Academy of Science and 
      Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.
FAU - Vo, Hoang D M
AU  - Vo HD
AD  - Institute of Applied Materials Science, Vietnam Academy of Science and 
      Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.
FAU - Vo, Nguyen P
AU  - Vo NP
AD  - Medical and Pharmaceutical University, 41 Dinh Tien Hoang Street, District 1, Ho 
      Chi Minh, Viet Nam.
FAU - Kudryashova, Kseniya S
AU  - Kudryashova KS
AD  - Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Moscow 
      119992, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian 
      Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
FAU - Nekrasova, Oksana V
AU  - Nekrasova OV
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
FAU - Feofanov, Alexey V
AU  - Feofanov AV
AD  - Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Moscow 
      119992, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian 
      Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
FAU - Kirpichnikov, Mikhail P
AU  - Kirpichnikov MP
AD  - Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Moscow 
      119992, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian 
      Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
FAU - Andreeva, Tatyana V
AU  - Andreeva TV
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
FAU - Serebryakova, Marina V
AU  - Serebryakova MV
AD  - Belozersky Institute for Physico-Chemical Biology, Lomonosov Moscow State 
      University, Moscow 119992, Russia.
FAU - Tsetlin, Victor I
AU  - Tsetlin VI
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
FAU - Utkin, Yuri N
AU  - Utkin YN
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia; OOO Syneuro, ul. 
      Miklukho-Maklaya 16/10, Moscow 117997, Russia. Electronic address: 
      utkin@mx.ibch.ru.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131101
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Analgesics)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Kv1.3 Potassium Channel)
RN  - 0 (Scorpion Venoms)
RN  - 0 (hetlaxin, Heterometrus laoticus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Analgesics/*pharmacology
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Chemical Fractionation
MH  - Chromatography, Reverse-Phase
MH  - Kv1.3 Potassium Channel/*metabolism
MH  - Lethal Dose 50
MH  - Mice
MH  - Molecular Sequence Data
MH  - Scorpion Venoms/genetics/pharmacology/*toxicity
MH  - Scorpions/*chemistry
MH  - Sequence Analysis, Protein
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Statistics, Nonparametric
MH  - Time Factors
MH  - Toxicity Tests, Acute
MH  - Vietnam
OTO - NOTNLM
OT  - Biological activity
OT  - Heterometrus laoticus
OT  - Potassium channel
OT  - Scorpion
OT  - Toxicity
OT  - Venom
EDAT- 2013/11/06 06:00
MHDA- 2014/08/20 06:00
CRDT- 2013/11/06 06:00
PHST- 2013/06/04 00:00 [received]
PHST- 2013/09/14 00:00 [revised]
PHST- 2013/10/24 00:00 [accepted]
PHST- 2013/11/06 06:00 [entrez]
PHST- 2013/11/06 06:00 [pubmed]
PHST- 2014/08/20 06:00 [medline]
AID - S0041-0101(13)00413-3 [pii]
AID - 10.1016/j.toxicon.2013.10.027 [doi]
PST - ppublish
SO  - Toxicon. 2014 Jan;77:40-8. doi: 10.1016/j.toxicon.2013.10.027. Epub 2013 Nov 1.