PMID- 24190256
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131106
LR  - 20211021
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 87
IP  - 3
DP  - 1993 Nov
TI  - Identification of RAPD markers linked to genetic factors controlling the milling 
      energy requirement of barley.
PG  - 314-20
LID - 10.1007/BF01184917 [doi]
AB  - Doubled haploid (DH) populations of barley have been used in combination with 
      PCR-based polymorphic-assay procedures to identify molecular markers linked to 
      genes controlling the milling energy requirement of the grain. Milling energy 
      (ME) is a quantitative trait and locating individual quantitative trait loci 
      (QTLs) involved the construction of bulks by combining DNA from DH families 
      representing the extreme members of the distribution for ME. In addition, the 
      individuals had alternative alleles at theRrn2 locus that has previously been 
      shown to be linked to an ME QTL. The DNA bulks were screened with Randomly 
      Amplified Polymorphic DNA (RAPD) markers and polymorphic amplification products 
      tested for linkage to genes influencing the expression of ME in a DH population. 
      Several markers were identified which are linked to a QTL controlling ME and the 
      recombination fraction determined by maximum likelihood procedures. The results 
      indicate that DHs in combination with RAPDs and bulked segregant analysis provide 
      an efficient method for locating QTLs in barely. Furthermore, this approach is 
      applicable to mapping other QTLs in a range of organisms from which DH or 
      recombinant inbred lines can be extracted.
FAU - Chalmers, K J
AU  - Chalmers KJ
AD  - Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, Scotland, UK.
FAU - Barua, U M
AU  - Barua UM
FAU - Hackett, C A
AU  - Hackett CA
FAU - Thomas
AU  - Thomas
FAU - Waugh, R
AU  - Waugh R
FAU - Powell, W
AU  - Powell W
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
CRDT- 2013/11/06 06:00
PHST- 1992/12/30 00:00 [received]
PHST- 1993/03/29 00:00 [accepted]
PHST- 2013/11/06 06:00 [entrez]
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
AID - 10.1007/BF01184917 [doi]
PST - ppublish
SO  - Theor Appl Genet. 1993 Nov;87(3):314-20. doi: 10.1007/BF01184917.