PMID- 24190435
OWN - NLM
STAT- MEDLINE
DCOM- 20140812
LR  - 20221207
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 33
IP  - 1
DP  - 2014 Jan
TI  - Inhibition of IL-32 and TSLP production through the attenuation of caspase-1 
      activation in an animal model of allergic rhinitis by Naju Jjok (Polygonum 
      tinctorium).
PG  - 142-50
LID - 10.3892/ijmm.2013.1548 [doi]
AB  - In this study, we investigated the effects of Naju Jjok (Polygonum tinctorium 
      Lour., NJJ) on interleukin (IL)-32 and thymic stromal lymphopoietin (TSLP) levels 
      associated with allergic rhinitis (AR). Using female BALB/c mice, we created an 
      animal model of ovalbumin (OVA)-induced AR. Prior to the callenge with OVA, the 
      mice were administered, either nasally or orally with NJJ. In addition, we also 
      used the eosinophilic cells line, Eol-1, stimulated with granulocyte‑macrophage 
      colony-stimulation factor (GM-CSF). The mRNA and protein levels of inflammatory 
      cytokines and markers [interleukin (IL)-32, IL-4, macrophage-inflammatory 
      protein-2 (MIP-2), intercellular adhesion molecule-1 (ICAM-1), and 
      cyclooxygenase-2 (COX-2)] were measured by RT-PCR and western blot analysis, 
      respectively and serum levels were measured by ELISA. The increased levels of 
      IL-32 in the mice with AR and in the stimulated eosinophilic cell line, Eol-1, 
      were significantly reduced by NJJ. TSLP levels were also decreased following the 
      oral administration of NJJ. Mice orally administered NJJ showed markedly 
      alleviated clinical symptoms, such as a reduced number of nasal rubs, decreased 
      spleen weight, decreased serum immunoglobulin E (IgE) levels and decreased serum 
      histamine levels. The oral administration of NJJ significantly decreased the IL-4 
      levels, while increasing the interferon-gamma levels in the spleen. The increased 
      number of eosinophils and mast cells infiltrating the nasal mucosal tissue of the 
      mice with AR were decreased following the oral administration of NJJ. NJJ 
      effectively attenuated caspase-1 activity in the mice with AR and in the 
      stimulated Eol-1 cells. The oral administration of NJJ significantly reduced the 
      levels of inflammatory markers, such as MIP-2, ICAM-1 and COX-2. Furthermore, the 
      intranasal administration of NJJ significantly reduced the early phase response 
      to allergen exposure, such as nasal rubs, IgE production and histamine release, 
      as well as the late phase responses, such as the expression of inflammatory 
      markers. In conclusion, these data demonstrate that NJJ may play a regulatory 
      role in nasal inflammation.
FAU - Jeong, Hyun-Ja
AU  - Jeong HJ
AD  - Biochip Research Center and Inflammatory Diseases Research Center, Hoseo 
      University, Asan, Chungnam 336-795, Republic of Korea.
FAU - Oh, Hyun-A
AU  - Oh HA
FAU - Lee, Byung-Joo
AU  - Lee BJ
FAU - Kim, Hyung-Min
AU  - Kim HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131105
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Cxcl2 protein, mouse)
RN  - 0 (Cytokines)
RN  - 0 (Icam1 protein, mouse)
RN  - 0 (Interleukins)
RN  - 0 (Plant Extracts)
RN  - 0 (interleukin-32, mouse)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 3.4.22.36 (Caspase 1)
RN  - GT0IL38SP4 (Thymic Stromal Lymphopoietin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Caspase 1/genetics/*metabolism
MH  - Cell Line
MH  - Chemokine CXCL2/genetics/metabolism
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Cytokines/*metabolism
MH  - Disease Models, Animal
MH  - Eosinophils/drug effects/metabolism
MH  - Female
MH  - Histamine/blood
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Interferon-gamma/metabolism
MH  - Interleukins/genetics/*metabolism
MH  - Mast Cells/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/adverse effects
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Polygonum/*chemistry
MH  - Rhinitis, Allergic
MH  - Rhinitis, Allergic, Perennial/*drug therapy
MH  - Spleen/drug effects/metabolism
MH  - Thymic Stromal Lymphopoietin
EDAT- 2013/11/06 06:00
MHDA- 2014/08/13 06:00
CRDT- 2013/11/06 06:00
PHST- 2013/07/27 00:00 [received]
PHST- 2013/10/18 00:00 [accepted]
PHST- 2013/11/06 06:00 [entrez]
PHST- 2013/11/06 06:00 [pubmed]
PHST- 2014/08/13 06:00 [medline]
AID - 10.3892/ijmm.2013.1548 [doi]
PST - ppublish
SO  - Int J Mol Med. 2014 Jan;33(1):142-50. doi: 10.3892/ijmm.2013.1548. Epub 2013 Nov 
      5.