PMID- 24190493 OWN - NLM STAT- MEDLINE DCOM- 20140826 LR - 20211021 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 41 IP - 1 DP - 2014 Jan TI - Antineoplastic and immunomodulatory effect of polyphenolic components of Achyranthes aspera (PCA) extract on urethane induced lung cancer in vivo. PG - 179-91 LID - 10.1007/s11033-013-2850-6 [doi] AB - Polyphenolic compounds of Achyranthes aspera (PCA) extract is evaluated for anti-cancerous and cytokine based immunomodulatory effects. The PCA extract contains known components of phenolic acid and flavonoids such as mixture of quinic acid, chlorogenic acid, kaempferol, quercetin and chrysin along with many unknown components. PCA has been orally feed to urethane (ethyl carbamate) primed lung cancerous mice at a dosage of 100 mg/kg body weight for 30 consecutive days. 100 mg powder of A. aspera contains 2.4 mg phenolic acid and 1.1 mg flavonoid (2:1 ratio). Enhanced activities and expression of antioxidant enzymes GST, GR, CAT, SOD, while down regulated expression and activation of LDH enzymes in PCA feed urethane primed lung cancerous tissues as compared to PCA non-feed urethane primed lung cancerous tissues were observed. PCA feed urethane primed lung tissues showed down regulated expression of pro-inflammatory cytokines IL-1beta, IL-6 and TNF-alpha along with TFs, NF-kappaB and Stat3 while the expression of pro-apoptotic proteins Bax and p53 were enhanced in PCA feed urethane primed lung tissues. FTIR and CD spectroscopy data revealed that PCA resisted the urethane mediated conformational changes of DNA which is evident by the shift in guanine and thymine bands in FTIR from 1,708 to 1,711 cm(-1) and 1,675 to 1,671 cm(-1), respectively in PCA feed urethane primed lung cancerous tissues DNA in comparison to urethane primed lung cancerous tissues DNA. The present study suggests that PCA components have synergistic anti-cancerous and cytokine based immunomodulatory role and DNA conformation restoring effects. However, more research is required to show the effects of each component separately and in combination for effective therapeutic use to cure and prevent lung cancer including other cancers. FAU - Narayan, Chandradeo AU - Narayan C AD - School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, 221 005, UP, India. FAU - Kumar, Arvind AU - Kumar A LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131105 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - 0 (Antineoplastic Agents, Phytogenic) RN - 0 (Cytokines) RN - 0 (Immunologic Factors) RN - 0 (Plant Extracts) RN - 0 (Polyphenols) RN - 3IN71E75Z5 (Urethane) RN - 9007-49-2 (DNA) RN - EC 1.1.1.27 (L-Lactate Dehydrogenase) RN - EC 1.11.1.6 (Catalase) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - EC 1.8.1.7 (Glutathione Reductase) RN - EC 2.5.1.18 (Glutathione Transferase) SB - IM MH - Achyranthes/*chemistry MH - Animals MH - Antineoplastic Agents, Phytogenic/isolation & purification/*pharmacology MH - Catalase/genetics/metabolism MH - Cytokines/genetics/metabolism MH - DNA/chemistry/genetics MH - Drug Screening Assays, Antitumor MH - Female MH - Glutathione Reductase/genetics/metabolism MH - Glutathione Transferase/genetics/metabolism MH - Immunologic Factors/isolation & purification/*pharmacology MH - L-Lactate Dehydrogenase/genetics/metabolism MH - Lung Neoplasms/chemically induced/*drug therapy MH - Male MH - Mice MH - Mice, Inbred BALB C MH - Plant Extracts/isolation & purification/*pharmacology MH - Polyphenols/isolation & purification/*pharmacology MH - Spectroscopy, Fourier Transform Infrared MH - Superoxide Dismutase/genetics/metabolism MH - Transcription, Genetic/drug effects MH - Urethane EDAT- 2013/11/06 06:00 MHDA- 2014/08/27 06:00 CRDT- 2013/11/06 06:00 PHST- 2012/12/18 00:00 [received] PHST- 2013/10/29 00:00 [accepted] PHST- 2013/11/06 06:00 [entrez] PHST- 2013/11/06 06:00 [pubmed] PHST- 2014/08/27 06:00 [medline] AID - 10.1007/s11033-013-2850-6 [doi] PST - ppublish SO - Mol Biol Rep. 2014 Jan;41(1):179-91. doi: 10.1007/s11033-013-2850-6. Epub 2013 Nov 5.