PMID- 2419060
OWN - NLM
STAT- MEDLINE
DCOM- 19860331
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 7
IP  - 2
DP  - 1986 Mar
TI  - Isolation of viable type II alveolar epithelial cells by flow cytometry.
PG  - 157-62
AB  - We developed a new method for isolating viable type II cells from fractionated 
      and unfractionated lung cell suspensions by flow cytometry using acridine orange 
      (AO). Fischer-344 rat lungs were dispersed into single-cell suspensions by a 
      technique that yields a high number of cells (4-5 X 10(8) cells/lung, congruent 
      to 85% viable), congruent to 11% of which are type II cells. Elutriated fractions 
      from the lung cell preparation and parent, unfractionated cell suspensions were 
      incubated with 1.0-0.02 micrograms/ml AO and analyzed by flow cytometry. 
      Parameters analyzed included axial light loss (ALL) and red fluorescence (RF). 
      Based on their unique RF, attributable to AO staining of type II cell lamellar 
      bodies, and their ALL characteristics, type II pneumocytes were sorted from 
      elutriated fractions to greater than 95% purity. Using the same approach, type II 
      pneumocytes were sorted from unfractionated lung cell suspensions at greater than 
      or equal to 85% purity. The viabilities of the type II alveolar epithelial cells 
      isolated by this method range from 85% to 95%, and the ultrastructural features 
      of the sorted cells were unaltered by AO labeling or sorting.
FAU - Wilson, J S
AU  - Wilson JS
FAU - Steinkamp, J A
AU  - Steinkamp JA
FAU - Lehnert, B E
AU  - Lehnert BE
LA  - eng
GR  - P41-RK 0125-02/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cell Separation
MH  - Flow Cytometry/*methods
MH  - Male
MH  - Microscopy, Electron
MH  - Pulmonary Alveoli/*cytology/ultrastructure
MH  - Rats
MH  - Rats, Inbred F344
MH  - Staining and Labeling
EDAT- 1986/03/01 00:00
MHDA- 1986/03/01 00:01
CRDT- 1986/03/01 00:00
PHST- 1986/03/01 00:00 [pubmed]
PHST- 1986/03/01 00:01 [medline]
PHST- 1986/03/01 00:00 [entrez]
AID - 10.1002/cyto.990070206 [doi]
PST - ppublish
SO  - Cytometry. 1986 Mar;7(2):157-62. doi: 10.1002/cyto.990070206.