PMID- 24193102
OWN - NLM
STAT- MEDLINE
DCOM- 20140529
LR  - 20211021
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 80
DP  - 2013 Oct 21
TI  - Procedure for the development of multi-depth circular cross-sectional 
      endothelialized microchannels-on-a-chip.
PG  - e50771
LID - 10.3791/50771 [doi]
LID - 50771
AB  - Efforts have been focused on developing in vitro assays for the study of 
      microvessels because in vivo animal studies are more time-consuming, expensive, 
      and observation and quantification are very challenging. However, conventional in 
      vitro microvessel assays have limitations when representing in vivo microvessels 
      with respect to three-dimensional (3D) geometry and providing continuous fluid 
      flow. Using a combination of photolithographic reflowable photoresist technique, 
      soft lithography, and microfluidics, we have developed a multi-depth circular 
      cross-sectional endothelialized microchannels-on-a-chip, which mimics the 3D 
      geometry of in vivo microvessels and runs under controlled continuous perfusion 
      flow. A positive reflowable photoresist was used to fabricate a master mold with 
      a semicircular cross-sectional microchannel network. By the alignment and bonding 
      of the two polydimethylsiloxane (PDMS) microchannels replicated from the master 
      mold, a cylindrical microchannel network was created. The diameters of the 
      microchannels can be well controlled. In addition, primary human umbilical vein 
      endothelial cells (HUVECs) seeded inside the chip showed that the cells lined the 
      inner surface of the microchannels under controlled perfusion lasting for a time 
      period between 4 days to 2 weeks.
FAU - Li, Xiang
AU  - Li X
AD  - Lane Department of Computer Science and Electrical Engineering, West Virginia 
      University.
FAU - Mearns, Samantha Marie
AU  - Mearns SM
FAU - Martins-Green, Manuela
AU  - Martins-Green M
FAU - Liu, Yuxin
AU  - Liu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Video-Audio Media
DEP - 20131021
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Dimethylpolysiloxanes)
SB  - IM
MH  - Dimethylpolysiloxanes/chemistry
MH  - Human Umbilical Vein Endothelial Cells/*cytology
MH  - Humans
MH  - *Lab-On-A-Chip Devices
MH  - Microfluidic Analytical Techniques
MH  - Microvessels/*cytology
PMC - PMC3947964
EDAT- 2013/11/07 06:00
MHDA- 2014/05/30 06:00
PMCR- 2015/10/21
CRDT- 2013/11/07 06:00
PHST- 2013/11/07 06:00 [entrez]
PHST- 2013/11/07 06:00 [pubmed]
PHST- 2014/05/30 06:00 [medline]
PHST- 2015/10/21 00:00 [pmc-release]
AID - 50771 [pii]
AID - 10.3791/50771 [doi]
PST - epublish
SO  - J Vis Exp. 2013 Oct 21;(80):e50771. doi: 10.3791/50771.