PMID- 24193167
OWN - NLM
STAT- MEDLINE
DCOM- 20140207
LR  - 20151119
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 162
IP  - 4
DP  - 2013
TI  - The relevance of basophil allergen sensitivity testing to distinguish between 
      severe and mild peanut-allergic children.
PG  - 310-7
LID - 10.1159/000355199 [doi]
AB  - BACKGROUND: Peanut sensitization is common in children. However, it is difficult 
      to assess which children will react mildly and which severely. This study 
      evaluated the relevance of basophil allergen sensitivity testing to distinguish 
      the severity of peanut allergy in children. METHODS: Twenty-seven 
      peanut-sensitized children with symptoms varying from mild symptoms to severe 
      anaphylaxis underwent peanut CD63 dose-response curve analysis with the inclusion 
      of basophil allergen sensitivity calculation (CD-sens) and peanut component 
      immunoglobulin E (IgE) testing. RESULTS: Eleven children who had experienced 
      anaphylaxis to peanuts showed a markedly higher peanut CD63 response at 
      submaximal allergen concentrations and CD-sens (median 1,667 vs. 0.5; p < 0.0001) 
      than 16 children who experienced a milder reaction. Furthermore, a negative or 
      low CD-sens to peanuts unambiguously excluded anaphylactic peanut allergy. 
      Children with anaphylaxis have higher levels of Ara h 1, 2, 3 and 9 IgE, but 
      comparable levels of IgE to Ara h 8 and whole-peanut extract. The diagnostic 
      specificity calculated with a receiver operating characteristic analysis reached 
      100% for CD-sens and 73% for Ara h 2. CONCLUSIONS: We demonstrated that severe 
      peanut allergy is significantly associated with higher basophil allergen 
      sensitivity. This cellular test should facilitate a more accurate diagnosis of 
      peanut allergy.
CI  - (c) 2013 S. Karger AG, Basel.
FAU - Homsak, Matjaz
AU  - Homsak M
AD  - Department of Pediatrics, University Medical Center, Maribor, Slovenia.
FAU - Silar, Mira
AU  - Silar M
FAU - Berce, Vojko
AU  - Berce V
FAU - Tomazin, Maja
AU  - Tomazin M
FAU - Skerbinjek-Kavalar, Maja
AU  - Skerbinjek-Kavalar M
FAU - Celesnik, Nina
AU  - Celesnik N
FAU - Kosnik, Mitja
AU  - Kosnik M
FAU - Korosec, Peter
AU  - Korosec P
LA  - eng
PT  - Journal Article
DEP - 20131025
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Allergens/genetics/*immunology
MH  - Arachis/*immunology
MH  - Basophils/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Peanut Hypersensitivity/*diagnosis/*immunology/physiopathology
MH  - Recombinant Proteins/genetics/metabolism
EDAT- 2013/11/07 06:00
MHDA- 2014/02/08 06:00
CRDT- 2013/11/07 06:00
PHST- 2013/01/07 00:00 [received]
PHST- 2013/08/14 00:00 [accepted]
PHST- 2013/11/07 06:00 [entrez]
PHST- 2013/11/07 06:00 [pubmed]
PHST- 2014/02/08 06:00 [medline]
AID - 000355199 [pii]
AID - 10.1159/000355199 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2013;162(4):310-7. doi: 10.1159/000355199. Epub 2013 
      Oct 25.