PMID- 24194897
OWN - NLM
STAT- MEDLINE
DCOM- 20150224
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 10
DP  - 2013
TI  - Down-regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal 
      squamous cell carcinoma by targeting APC.
PG  - e77829
LID - 10.1371/journal.pone.0077829 [doi]
LID - e77829
AB  - miRNAs regulate gene expression and are key mediators of tumourigenesis. miR-129 
      has diverse effects in tumours, but its role in laryngeal squamous cell carcinoma 
      (LSCC) remains unknown. This article focuses on the role of miR-129-5p in LSCC. 
      We show miR-129-5p is upregulated in primary LSCC tumours and correlated with 
      advanced disease. Down-regulating miR-129-5p suppressed cell proliferation and 
      migration, and caused cell cycle arrest in Hep-2 cell lines. Downregulation of 
      miR-129-5p alone is sufficient to induce apoptosis both in vivo and in vitro. 
      Moreover, the growth of LSCC xenograft exposed to miR-129-5p antisense 
      oligonucleotides (ASO) in BALB/c mice was markedly inhibited. In addition, we 
      found that miR-129-5p targeted adenomatous polyposis coli (APC) to release 
      inhibition of Wnt signalling causing cell growth and tumourigenesis. Our results 
      suggest miR-129-5p functions as an oncogene in LSCC by repressing APC and is a 
      potential therapeutic target for LSCC.
FAU - Li, MingHua
AU  - Li M
AD  - Services of Head and Neck Surgery, department of Otolaryngology-Head and Neck 
      Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 
      China.
FAU - Tian, LinLi
AU  - Tian L
FAU - Wang, Lin
AU  - Wang L
FAU - Yao, HongChao
AU  - Yao H
FAU - Zhang, JiaRui
AU  - Zhang J
FAU - Lu, JianGuang
AU  - Lu J
FAU - Sun, YaNan
AU  - Sun Y
FAU - Gao, Xu
AU  - Gao X
FAU - Xiao, Hui
AU  - Xiao H
FAU - Liu, Ming
AU  - Liu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131023
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Adenomatous Polyposis Coli Protein)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn129 microRNA, human)
RN  - 0 (Oligonucleotides, Antisense)
SB  - IM
MH  - Adenomatous Polyposis Coli Protein/*metabolism
MH  - Animals
MH  - Apoptosis/genetics
MH  - Carcinoma, Squamous Cell/genetics/*metabolism
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Laryngeal Neoplasms/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*metabolism
MH  - Oligonucleotides, Antisense/genetics
PMC - PMC3806837
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/11/07 06:00
MHDA- 2015/02/25 06:00
PMCR- 2013/10/23
CRDT- 2013/11/07 06:00
PHST- 2013/07/02 00:00 [received]
PHST- 2013/09/11 00:00 [accepted]
PHST- 2013/11/07 06:00 [entrez]
PHST- 2013/11/07 06:00 [pubmed]
PHST- 2015/02/25 06:00 [medline]
PHST- 2013/10/23 00:00 [pmc-release]
AID - PONE-D-13-27270 [pii]
AID - 10.1371/journal.pone.0077829 [doi]
PST - epublish
SO  - PLoS One. 2013 Oct 23;8(10):e77829. doi: 10.1371/journal.pone.0077829. 
      eCollection 2013.