PMID- 24200387
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20211203
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 23
IP  - 4
DP  - 2014 Feb 15
TI  - The miR-7 identified from collagen biomaterial-based three-dimensional cultured 
      cells regulates neural stem cell differentiation.
PG  - 393-405
LID - 10.1089/scd.2013.0342 [doi]
AB  - Increasing evidence suggests that three-dimensional (3D) cultures provide more 
      appropriate microenvironments to control stem cell response compared with 
      traditional two-dimensional (2D) cultures. However, the molecular mechanism 
      involved in 3D cultured stem cells is not well known. Several microRNAs whose 
      target genes involved in the regulation of self-renewal and differentiation of 
      stem cells were found to be downregulated in 3D cultured PA-1 cells. Among them, 
      miR-7 was predicted to target Kruppel-like factor 4 (Klf4), a key gene for 
      self-renewal of neural stem cells (NSCs). We showed that the differentiation of 
      NSCs was inhibited in 3D collagen scaffolds compared with 2D cultured cells. The 
      quantitative real-time PCR (qPCR) analysis indicated that the expression of miR-7 
      and Klf4 changed significantly in 2D cultures, whereas the expression stability 
      of miR-7 and Klf4 was detected in 3D cultures. Using luciferase assay and western 
      blot, Klf4 was identified as a target of miR-7 indicating that miR-7 plays a 
      critical role in maintaining the self-renewal capacity through a Klf4-dependent 
      mechanism in 3D cultured cells. Thus, the collagen scaffold-based 3D cell 
      cultures may provide a platform to reveal the regulatory mechanism of cell 
      regulators, which are difficult to find in traditional 2D cell cultures.
FAU - Cui, Yi
AU  - Cui Y
AD  - 1 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology , Chinese Academy of Sciences, Beijing, China .
FAU - Xiao, Zhifeng
AU  - Xiao Z
FAU - Chen, Tong
AU  - Chen T
FAU - Wei, Jianshu
AU  - Wei J
FAU - Chen, Lei
AU  - Chen L
FAU - Liu, Lijun
AU  - Liu L
FAU - Chen, Bing
AU  - Chen B
FAU - Wang, Xiujie
AU  - Wang X
FAU - Li, Xiaoran
AU  - Li X
FAU - Dai, Jianwu
AU  - Dai J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131221
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (3' Untranslated Regions)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MIRN7 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression
MH  - Humans
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/*genetics/metabolism
MH  - MicroRNAs/*physiology
MH  - Neural Stem Cells/*physiology
MH  - RNA Interference
MH  - Transcriptome
PMC - PMC3920754
EDAT- 2013/11/10 06:00
MHDA- 2014/10/01 06:00
PMCR- 2015/02/15
CRDT- 2013/11/09 06:00
PHST- 2013/11/09 06:00 [entrez]
PHST- 2013/11/10 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
PHST- 2015/02/15 00:00 [pmc-release]
AID - 10.1089/scd.2013.0342 [pii]
AID - 10.1089/scd.2013.0342 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2014 Feb 15;23(4):393-405. doi: 10.1089/scd.2013.0342. Epub 2013 
      Dec 21.