PMID- 24201816
OWN - NLM
STAT- MEDLINE
DCOM- 20140929
LR  - 20240323
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 38
IP  - 1
DP  - 2014 Jan-Feb
TI  - An enhanced throughput method for quantification of sulfur mustard adducts to 
      human serum albumin via isotope dilution tandem mass spectrometry.
PG  - 8-15
LID - 10.1093/jat/bkt088 [doi]
AB  - Here, we report an enhanced throughput method for the diagnosis of human exposure 
      to sulfur mustard. A hydroxyethylthioethyl (HETE) ester-adducted tripeptide, 
      produced by pronase digestion of human serum albumin, was selected as the 
      quantitative exposure biomarker. Cibacron Blue enrichment was developed from an 
      established cartridge method into a 96-well plate format, increasing throughput 
      and ruggedness. This new method decreased sample volume 2.5-fold. Addition of a 
      precipitation and solid-phase extraction concentration step increased the 
      sensitivity of the method. With the conversion to a 96-well plate and 
      optimization of chromatography, the method resulted in a 3-fold decrease in 
      analysis time. Inclusion of a confirmation ion has increased specificity. The 
      method was found to be linear between 0.050 and 50 microM sulfur mustard exposure 
      with a precision for both quality control samples of </=6.5% relative standard 
      deviation and an accuracy of >96%. The limit of detection (3So) was calculated to 
      be  approximately 0.0048 microM, an exposure value similar to that of the HETE-albumin adduct 
      method first described by Noort and co-workers (Noort et al., 1999; Noort el al., 
      2004) which used protein precipitation to isolate albumin. A convenience set of 
      124 plasma samples from healthy unexposed individuals was analyzed using this 
      method to assess background levels of exposure to sulfur mustard; no positive 
      results were detected.
FAU - Andacht, Tracy M
AU  - Andacht TM
AD  - 1Emergency Response Branch, Division of Laboratory Sciences, National Center for 
      Environmental Health, Centers for Disease Control and Prevention, 4770 Buford 
      Highway, NE, Atlanta, GA 30341, USA.
FAU - Pantazides, Brooke G
AU  - Pantazides BG
FAU - Crow, Brian S
AU  - Crow BS
FAU - Fidder, Alex
AU  - Fidder A
FAU - Noort, Daan
AU  - Noort D
FAU - Thomas, Jerry D
AU  - Thomas JD
FAU - Blake, Thomas A
AU  - Blake TA
FAU - Johnson, Rudolph C
AU  - Johnson RC
LA  - eng
GR  - CC999999/Intramural CDC HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20131107
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Biomarkers)
RN  - 0 (Serum Albumin)
RN  - T8KEC9FH9P (Mustard Gas)
SB  - IM
MH  - Biomarkers/blood
MH  - Calibration
MH  - Environmental Exposure/*analysis
MH  - High-Throughput Screening Assays/*methods
MH  - Humans
MH  - Mustard Gas/*toxicity
MH  - Proteomics
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Serum Albumin/*chemistry
MH  - Solid Phase Extraction
MH  - Specimen Handling
MH  - Tandem Mass Spectrometry/*methods
PMC - PMC4539155
MID - NIHMS714681
OID - NLM: HHSPA714681
EDAT- 2013/11/10 06:00
MHDA- 2014/09/30 06:00
PMCR- 2015/08/17
CRDT- 2013/11/09 06:00
PHST- 2013/11/09 06:00 [entrez]
PHST- 2013/11/10 06:00 [pubmed]
PHST- 2014/09/30 06:00 [medline]
PHST- 2015/08/17 00:00 [pmc-release]
AID - bkt088 [pii]
AID - 10.1093/jat/bkt088 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2014 Jan-Feb;38(1):8-15. doi: 10.1093/jat/bkt088. Epub 2013 Nov 
      7.