PMID- 24204675
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 10
DP  - 2013
TI  - Steroid-associated hip joint collapse in bipedal emus.
PG  - e76797
LID - 10.1371/journal.pone.0076797 [doi]
LID - e76797
AB  - In this study we established a bipedal animal model of steroid-associated hip 
      joint collapse in emus for testing potential treatment protocols to be developed 
      for prevention of steroid-associated joint collapse in preclinical settings. Five 
      adult male emus were treated with a steroid-associated osteonecrosis (SAON) 
      induction protocol using combination of pulsed lipopolysaccharide (LPS) and 
      methylprednisolone (MPS). Additional three emus were used as normal control. 
      Post-induction, emu gait was observed, magnetic resonance imaging (MRI) was 
      performed, and blood was collected for routine examination, including testing 
      blood coagulation and lipid metabolism. Emus were sacrificed at week 24 
      post-induction, bilateral femora were collected for micro-computed tomography 
      (micro-CT) and histological analysis. Asymmetric limping gait and abnormal MRI 
      signals were found in steroid-treated emus. SAON was found in all emus with a 
      joint collapse incidence of 70%. The percentage of neutrophils (Neut %) and 
      parameters on lipid metabolism significantly increased after induction. Micro-CT 
      revealed structure deterioration of subchondral trabecular bone. Histomorphometry 
      showed larger fat cell fraction and size, thinning of subchondral plate and 
      cartilage layer, smaller osteoblast perimeter percentage and less blood vessels 
      distributed at collapsed region in SAON group as compared with the normal 
      controls. Scanning electron microscope (SEM) showed poor mineral matrix and more 
      osteo-lacunae outline in the collapsed region in SAON group. The combination of 
      pulsed LPS and MPS developed in the current study was safe and effective to 
      induce SAON and deterioration of subchondral bone in bipedal emus with subsequent 
      femoral head collapse, a typical clinical feature observed in patients under 
      pulsed steroid treatment. In conclusion, bipedal emus could be used as an 
      effective preclinical experimental model to evaluate potential treatment 
      protocols to be developed for prevention of ON-induced hip joint collapse in 
      patients.
FAU - Zheng, Li-Zhen
AU  - Zheng LZ
AD  - Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong, 
      Hong Kong SAR, China.
FAU - Liu, Zhong
AU  - Liu Z
FAU - Lei, Ming
AU  - Lei M
FAU - Peng, Jiang
AU  - Peng J
FAU - He, Yi-Xin
AU  - He YX
FAU - Xie, Xin-Hui
AU  - Xie XH
FAU - Man, Chi-Wai
AU  - Man CW
FAU - Huang, Le
AU  - Huang L
FAU - Wang, Xin-Luan
AU  - Wang XL
FAU - Fong, Daniel Tik-Pui
AU  - Fong DT
FAU - Xiao, De-Ming
AU  - Xiao DM
FAU - Wang, Da-Ping
AU  - Wang DP
FAU - Chen, Yang
AU  - Chen Y
FAU - Feng, Jian Q
AU  - Feng JQ
FAU - Liu, Ying
AU  - Liu Y
FAU - Zhang, Ge
AU  - Zhang G
FAU - Qin, Ling
AU  - Qin L
LA  - eng
GR  - R01 DE018486/DE/NIDCR NIH HHS/United States
GR  - DE018486/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20131021
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Lipopolysaccharides)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Dromaiidae
MH  - Femur/diagnostic imaging/pathology/ultrastructure
MH  - Hip Joint/diagnostic imaging/*pathology/physiopathology
MH  - Humans
MH  - Lipid Metabolism
MH  - Lipopolysaccharides
MH  - Magnetic Resonance Imaging
MH  - Methylprednisolone
MH  - Microscopy, Electron, Scanning
MH  - Neutrophils/pathology
MH  - Osteonecrosis/chemically induced/diagnostic imaging/*pathology
MH  - X-Ray Microtomography
PMC - PMC3804596
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/11/10 06:00
MHDA- 2014/08/27 06:00
PMCR- 2013/10/21
CRDT- 2013/11/09 06:00
PHST- 2013/04/17 00:00 [received]
PHST- 2013/08/28 00:00 [accepted]
PHST- 2013/11/09 06:00 [entrez]
PHST- 2013/11/10 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
PHST- 2013/10/21 00:00 [pmc-release]
AID - PONE-D-13-16173 [pii]
AID - 10.1371/journal.pone.0076797 [doi]
PST - epublish
SO  - PLoS One. 2013 Oct 21;8(10):e76797. doi: 10.1371/journal.pone.0076797. 
      eCollection 2013.